6b6j: Difference between revisions
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==Structure of profilin Art v4== | ==Structure of profilin Art v4== | ||
<StructureSection load='6b6j' size='340' side='right' caption='[[6b6j]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='6b6j' size='340' side='right'caption='[[6b6j]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6b6j]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6b6j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Artemisia_vulgaris Artemisia vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B6J FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6j OCA], [https://pdbe.org/6b6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b6j RCSB], [https://www.ebi.ac.uk/pdbsum/6b6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PROF1_ARTVU PROF1_ARTVU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Worldwide, more than one-third of the population suffers from allergies. A significant fraction of officially registered allergens originate from the profilin family of proteins. Profilins are small ubiquitous proteins which are found in plants, viruses and various eukaryotes including mammals. Although they are primarily regarded as minor allergens, profilins are important players in immunoglobulin E (IgE) cross-reactivity. However, in some populations profilins are recognized by IgE from at least 50% of patients allergic to a given allergen source. Cuc m 2.0101 is recognized by IgE in more than 80% of muskmelon-allergic patients. The recombinant isoallergen Cuc m 2.0101 was produced in significant quantities and its X-ray crystal structure was determined. In addition, a new Art v 4.0101 (mugwort profilin) structure was determined. The profilins Cuc m 2.0101 and Art v 4.0101 were compared in terms of their structure and thermal stability. Furthermore, structural similarities and IgE cross-reactivity between profilins from different sources are discussed to explain the molecular basis of various clinical syndromes involving this group of allergens. Special emphasis is placed on discussion of profilins' quaternary structures and their relation to biological function, as well as to protein allergenicity. Moreover, a potential impact of protein purification protocols on the structure of profilins is highlighted. | |||
Comparative structural and thermal stability studies of Cuc m 2.0101, Art v 4.0101 and other allergenic profilins.,Kapingidza AB, Pye SE, Hyduke N, Dolamore C, Pote S, Schlachter CR, Commins SP, Kowal K, Chruszcz M Mol Immunol. 2019 Jul 18;114:19-29. doi: 10.1016/j.molimm.2019.07.004. PMID:31326654<ref>PMID:31326654</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6b6j" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Profilin 3D Structures|Profilin 3D Structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Artemisia vulgaris]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Chruszcz M]] | ||
[[Category: | [[Category: Pote SS]] | ||
[[Category: | [[Category: Pye SE]] | ||
Latest revision as of 17:33, 4 October 2023
Structure of profilin Art v4Structure of profilin Art v4
Structural highlights
FunctionPublication Abstract from PubMedWorldwide, more than one-third of the population suffers from allergies. A significant fraction of officially registered allergens originate from the profilin family of proteins. Profilins are small ubiquitous proteins which are found in plants, viruses and various eukaryotes including mammals. Although they are primarily regarded as minor allergens, profilins are important players in immunoglobulin E (IgE) cross-reactivity. However, in some populations profilins are recognized by IgE from at least 50% of patients allergic to a given allergen source. Cuc m 2.0101 is recognized by IgE in more than 80% of muskmelon-allergic patients. The recombinant isoallergen Cuc m 2.0101 was produced in significant quantities and its X-ray crystal structure was determined. In addition, a new Art v 4.0101 (mugwort profilin) structure was determined. The profilins Cuc m 2.0101 and Art v 4.0101 were compared in terms of their structure and thermal stability. Furthermore, structural similarities and IgE cross-reactivity between profilins from different sources are discussed to explain the molecular basis of various clinical syndromes involving this group of allergens. Special emphasis is placed on discussion of profilins' quaternary structures and their relation to biological function, as well as to protein allergenicity. Moreover, a potential impact of protein purification protocols on the structure of profilins is highlighted. Comparative structural and thermal stability studies of Cuc m 2.0101, Art v 4.0101 and other allergenic profilins.,Kapingidza AB, Pye SE, Hyduke N, Dolamore C, Pote S, Schlachter CR, Commins SP, Kowal K, Chruszcz M Mol Immunol. 2019 Jul 18;114:19-29. doi: 10.1016/j.molimm.2019.07.004. PMID:31326654[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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