5w3d: Difference between revisions

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-'''Unreleased structure'''
-The entry 5w3d is ON HOLD  until Paper Publication
+==The structure of kinesin-14 wild-type Ncd-ADP dimer==
+<StructureSection load='5w3d' size='340' side='right'caption='[[5w3d]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5w3d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W3D FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.79&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3d OCA], [https://pdbe.org/5w3d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w3d RCSB], [https://www.ebi.ac.uk/pdbsum/5w3d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3d ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NCD_DROME NCD_DROME] NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.<ref>PMID:2146510</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Kinesin microtubule motor proteins play essential roles in division, including attaching chromosomes to spindles and crosslinking microtubules for spindle assembly. Human kinesin-14 KIFC1 is unique in that cancer cells with amplified centrosomes are dependent on the motor for viable division because of its ability to cluster centrosomes and form bipolar spindles, but it is not required for division in almost all normal cells. Screens for small molecule inhibitors of KIFC1 have yielded several candidates for further development, but obtaining structural data to determine their sites of binding has been difficult. Here we compare a previously unreported KIFC1 crystal structure with new structures of two closely related kinesin-14 proteins, Ncd and KIFC3, to determine the potential binding site of a known KIFC1 ATPase inhibitor, AZ82. We analyze the previously identified kinesin inhibitor binding sites and identify features of AZ82 that favor binding to one of the sites, the alpha4/alpha6 site. This selectivity can be explained by unique structural features of the KIFC1 alpha4/alpha6 binding site. These features may help improve the drug-like properties of AZ82 and other specific KIFC1 inhibitors.
-Authors: Park, H.W., Ma, Z., Chacko, J., Jiang, S.M., Robinson, R.C., Endow, S.A.
+Structural basis of small molecule ATPase inhibition of a human mitotic kinesin motor protein.,Park HW, Ma Z, Zhu H, Jiang S, Robinson RC, Endow SA Sci Rep. 2017 Nov 9;7(1):15121. doi: 10.1038/s41598-017-14754-6. PMID:29123223<ref>PMID:29123223</ref>
-Description: The structure of Kinesin-14 wild-type Ncd-AMPPNP dimer
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Endow, S.A]]
+<div class="pdbe-citations 5w3d" style="background-color:#fffaf0;"></div>
-[[Category: Ma, Z]]
+== References ==
-[[Category: Park, H.W]]
+<references/>
-[[Category: Robinson, R.C]]
+__TOC__
-[[Category: Chacko, J]]
+</StructureSection>
-[[Category: Jiang, S.M]]
+[[Category: Drosophila melanogaster]]
+[[Category: Large Structures]]
+[[Category: Chacko J]]
+[[Category: Endow SA]]
+[[Category: Jiang SM]]
+[[Category: Ma Z]]
+[[Category: Park HW]]
+[[Category: Robinson RC]]