5u91: Difference between revisions
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==Crystal structure of Tre/loxLTR complex== | |||
<StructureSection load='5u91' size='340' side='right'caption='[[5u91]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5u91]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U91 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.104Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u91 OCA], [https://pdbe.org/5u91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u91 RCSB], [https://www.ebi.ac.uk/pdbsum/5u91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u91 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
As part of the HIV infection cycle, viral DNA inserts into the genome of host cells such that the integrated DNA encoding the viral proteins is flanked by long terminal repeat (LTR) regions from the retrovirus. In an effort to develop novel genome editing techniques that safely excise HIV provirus from cells, Tre, an engineered version of Cre recombinase, was designed to target a 34-bp sequence within the HIV-1 LTR (loxLTR). The sequence targeted by Tre lacks the symmetry present in loxP, the natural DNA substrate for Cre. We report here the crystal structure of a catalytically inactive (Y324F) mutant of this engineered Tre recombinase in complex with the loxLTR DNA substrate. We also report that 17 of the 19 amino acid changes relative to Cre contribute to the altered specificity, even though many of these residues do not contact the DNA directly. We hypothesize that some mutations increase the flexibility of the Cre tetramer and that this, along with flexibility in the DNA, enable the engineered enzyme and DNA substrate to adopt complementary conformations. | |||
Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA.,Meinke G, Karpinski J, Buchholz F, Bohm A Nucleic Acids Res. 2017 Sep 19;45(16):9726-9740. doi: 10.1093/nar/gkx603. PMID:28934476<ref>PMID:28934476</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5u91" style="background-color:#fffaf0;"></div> | ||
[[Category: Bohm | |||
[[Category: Buchholz | ==See Also== | ||
[[Category: Karpinski | *[[Resolvase 3D structures|Resolvase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human immunodeficiency virus 1]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Bohm A]] | |||
[[Category: Buchholz F]] | |||
[[Category: Karpinski J]] | |||
[[Category: Meinke G]] | |||
Latest revision as of 16:23, 4 October 2023
Crystal structure of Tre/loxLTR complexCrystal structure of Tre/loxLTR complex
Structural highlights
Publication Abstract from PubMedAs part of the HIV infection cycle, viral DNA inserts into the genome of host cells such that the integrated DNA encoding the viral proteins is flanked by long terminal repeat (LTR) regions from the retrovirus. In an effort to develop novel genome editing techniques that safely excise HIV provirus from cells, Tre, an engineered version of Cre recombinase, was designed to target a 34-bp sequence within the HIV-1 LTR (loxLTR). The sequence targeted by Tre lacks the symmetry present in loxP, the natural DNA substrate for Cre. We report here the crystal structure of a catalytically inactive (Y324F) mutant of this engineered Tre recombinase in complex with the loxLTR DNA substrate. We also report that 17 of the 19 amino acid changes relative to Cre contribute to the altered specificity, even though many of these residues do not contact the DNA directly. We hypothesize that some mutations increase the flexibility of the Cre tetramer and that this, along with flexibility in the DNA, enable the engineered enzyme and DNA substrate to adopt complementary conformations. Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA.,Meinke G, Karpinski J, Buchholz F, Bohm A Nucleic Acids Res. 2017 Sep 19;45(16):9726-9740. doi: 10.1093/nar/gkx603. PMID:28934476[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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