5ti8: Difference between revisions
Jump to navigation
Jump to search
New page: '''Unreleased structure''' The entry 5ti8 is ON HOLD until Paper Publication Authors: Peat, T.S., Newman, J. Description: Crystal Structure of an aspartate aminotransferase from Pseudo... |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal Structure of an aspartate aminotransferase from Pseudomonas== | |||
<StructureSection load='5ti8' size='340' side='right'caption='[[5ti8]], [[Resolution|resolution]] 2.07Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5ti8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._M1 Pseudomonas sp. M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TI8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ti8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ti8 OCA], [https://pdbe.org/5ti8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ti8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ti8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ti8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/W5IS25_PSEUO W5IS25_PSEUO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The putrescine aminotransferase KES24511 from Pseudomonas sp. strain AAC was previously identified as an industrially relevant enzyme based on the discovery that it is able to promiscuously catalyse the transamination of 12-aminododecanoic acid. Here, the cloning, heterologous expression, purification and successful crystallization of the KES24511 protein are reported, which ultimately generated crystals adopting space group I2. The crystals diffracted X-rays to 2.07 A resolution and data were collected using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4a6t as the search model. | |||
Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.,Wilding M, Scott C, Newman J, Peat TS Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):29-35. doi:, 10.1107/S2053230X16019658. Epub 2017 Jan 1. PMID:28045391<ref>PMID:28045391</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5ti8" style="background-color:#fffaf0;"></div> | ||
[[Category: Newman | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas sp. M1]] | |||
[[Category: Newman J]] | |||
[[Category: Peat TS]] | |||
Latest revision as of 16:01, 4 October 2023
Crystal Structure of an aspartate aminotransferase from PseudomonasCrystal Structure of an aspartate aminotransferase from Pseudomonas
Structural highlights
FunctionPublication Abstract from PubMedThe putrescine aminotransferase KES24511 from Pseudomonas sp. strain AAC was previously identified as an industrially relevant enzyme based on the discovery that it is able to promiscuously catalyse the transamination of 12-aminododecanoic acid. Here, the cloning, heterologous expression, purification and successful crystallization of the KES24511 protein are reported, which ultimately generated crystals adopting space group I2. The crystals diffracted X-rays to 2.07 A resolution and data were collected using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4a6t as the search model. Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.,Wilding M, Scott C, Newman J, Peat TS Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):29-35. doi:, 10.1107/S2053230X16019658. Epub 2017 Jan 1. PMID:28045391[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||