5t6j: Difference between revisions

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 ==Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly==
-<StructureSection load='5t6j' size='340' side='right' caption='[[5t6j]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+<StructureSection load='5t6j' size='340' side='right'caption='[[5t6j]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5t6j]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T6J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T6J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5t6j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T6J FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t6j OCA], [http://pdbe.org/5t6j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t6j RCSB], [http://www.ebi.ac.uk/pdbsum/5t6j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t6j ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.752&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t6j OCA], [https://pdbe.org/5t6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t6j RCSB], [https://www.ebi.ac.uk/pdbsum/5t6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t6j ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref>  [[http://www.uniprot.org/uniprot/DSN1_YEAST DSN1_YEAST]] Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules.<ref>PMID:12455957</ref> <ref>PMID:14633972</ref>  [[http://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>
+[https://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.
+Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4):1014-1027.e12. doi: 10.1016/j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300<ref>PMID:27881300</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 5t6j" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Dimitrova, Y]]
+[[Category: Large Structures]]
-[[Category: Harrison, S C]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Jenni, S]]
+[[Category: Dimitrova Y]]
-[[Category: Khin, Y]]
+[[Category: Harrison SC]]
-[[Category: Valverde, R]]
+[[Category: Jenni S]]
-[[Category: Cell cycle]]
+[[Category: Khin Y]]
-[[Category: Chromosome]]
+[[Category: Valverde R]]
-[[Category: Complex]]
-[[Category: Kinetochore]]
-[[Category: Mind]]
-[[Category: Mis12]]
-[[Category: Mtw1]]
-[[Category: Ndc80]]
-[[Category: Nuf2]]
-[[Category: Segregation]]
-[[Category: Spc24]]
-[[Category: Spc25]]