Pyruvate-ferredoxin oxidoreductase: Difference between revisions

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Michal Harel, Alexander Berchansky

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-<Structure load='2c3o' size='400' frame='true' align='right' caption=' Pyruvate-ferredoxin oxidoredoxin dimer with Fe4S4 cluster complex with thiamin diphosphate and pyruvate [[2c3o]]' scene= />
+<StructureSection load='2c3o' size='350' side='right' scene='49/490113/Cv/1' caption='Pyruvate-ferredoxin oxidoredoxin dimer with Fe4S4 cluster complex with thiamine diphosphate and pyruvate [[2c3o]]'>
-<!--
+== Function ==
-Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
+'''Pyruvate-ferredoxin oxidoreductase''' (PFOR) is an enzyme of the fermentation cycle which catalyzes the oxidative decarboxylation of pyruvate to acetyl CoA and CO<sub>2</sub>.  This reaction provides the electron source for the reduction of ferredoxin<ref>PMID:10878009</ref>.  The reaction is CoA-dependent and contains thiamine diphosphate (TDP).  PFOR contains iron-sulfur clusters (Fe<sub>4</sub>S<sub>4</sub>).
-Or use the four-green-boxes-button to insert scrollable text adjacent
-to a Jmol applet. Check out the other buttons as well!
--->
-'''Pyruvate-ferredoxin oxidoreductase''' (PFOR) is an enzyme of the fermentation cycle which catalyzes the oxidative decarboxylation of pyruvate to acetyl CoA and CO2.  This reaction provides the electron source for the reduction of ferredoxin.  The reaction is CoA-dependent and contains thiamine diphosphate (TDP).  PFOR contains iron-sulfur clusters (Fe4S4).
+== Structural highlights ==
+The <scene name='49/490113/Cv/6'>active site of PFOR contains thiamine diphosphate and pyruvate</scene><ref>PMID:16472741</ref>. <scene name='49/490113/Cv/7'>Mg coordination site</scene>. Water molecules are shown as red spheres. <scene name='49/490113/Cv/8'>Fe4S4 cluster interactions</scene>. Each Fe atom interacts with S atom from cysteine.
+</StructureSection>
 ==3D structures of pyruvate-ferredoxin oxidoreductase==
-''Update June 2012''
+{{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
 [[1b0p]], [[2c3m]] – DaPFOR + TDP – ''Desulfovibrio africanus''<br />
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 [[2c3p]] - DaPFOR + TDP derivative<br />
 [[2c3u]] - DaPFOR + pyruvate + TDP derivative<br />
-[[2raa]] - PFOR γ subunit - ''Thermotoga maritima''
+[[7plm]] – DaPFOR + TPP – Cryo EM<br />
+[[2raa]] - PFOR γ subunit - ''Thermotoga maritima''<br />
+[[6cin]] – MtPFOR + TDP – ''Moorella thermoacetica''<br />
+[[6cio]], [[6cip]] – MtPFOR + TTP derivative <br />
+[[6ciq]] – MtPFOR + TTP + CoA <br />
+== References ==
+<references/>
 [[Category:Topic Page]]