2f03: Difference between revisions
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==Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)== | |||
<StructureSection load='2f03' size='340' side='right'caption='[[2f03]], [[Resolution|resolution]] 3.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2f03]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fimbriatus Streptomyces fimbriatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F03 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f03 OCA], [https://pdbe.org/2f03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f03 RCSB], [https://www.ebi.ac.uk/pdbsum/2f03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f03 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/T2S1_STRFI T2S1_STRFI] Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Many reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar. | |||
A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA.,Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:16308566<ref>PMID:16308566</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2f03" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
*[[Endonuclease 3D structures|Endonuclease 3D structures]] | |||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Aggarwal | __TOC__ | ||
[[Category: Vanamee | </StructureSection> | ||
[[Category: Viadiu | [[Category: Large Structures]] | ||
[[Category: Streptomyces fimbriatus]] | |||
[[Category: Aggarwal AK]] | |||
[[Category: Vanamee ES]] | |||
[[Category: Viadiu H]] | |||
Latest revision as of 13:26, 27 September 2023
Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)
Structural highlights
FunctionT2S1_STRFI Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9. Publication Abstract from PubMedMany reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar. A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA.,Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:16308566[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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