5d91: Difference between revisions
New page: '''Unreleased structure''' The entry 5d91 is ON HOLD Authors: Clarke, O.B., Tomasek, D.T., Jorge, C.D., Belcher Dufrisne, M., Kim, M., Banerjee, S., Rajashankar, K.R., Hendrickson, W.A.... |
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The | ==Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum== | ||
<StructureSection load='5d91' size='340' side='right'caption='[[5d91]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5d91]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304] and [https://en.wikipedia.org/wiki/Renibacterium_salmoninarum_ATCC_33209 Renibacterium salmoninarum ATCC 33209]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D91 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.501Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d91 OCA], [https://pdbe.org/5d91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d91 RCSB], [https://www.ebi.ac.uk/pdbsum/5d91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d91 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O27985_ARCFU O27985_ARCFU] [https://www.uniprot.org/uniprot/A9WSF5_RENSM A9WSF5_RENSM] Catalyzes the conjugation of the 1'-hydroxyl group of D-myo-inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.[HAMAP-Rule:MF_02241] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates and proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis is catalysed by CDP-alcohol phosphotransferases, transmembrane enzymes that use CDP-diacylglycerol as donor substrate for this reaction, and either inositol in eukaryotes or inositol phosphate in prokaryotes as the acceptor alcohol. Here we report the structures of a related enzyme, the phosphatidylinositol-phosphate synthase from Renibacterium salmoninarum, with and without bound CDP-diacylglycerol to 3.6 and 2.5 A resolution, respectively. These structures reveal the location of the acceptor site, and the molecular determinants of substrate specificity and catalysis. Functional characterization of the 40%-identical ortholog from Mycobacterium tuberculosis, a potential target for the development of novel anti-tuberculosis drugs, supports the proposed mechanism of substrate binding and catalysis. This work therefore provides a structural and functional framework to understand the mechanism of phosphatidylinositol-phosphate biosynthesis. | |||
Structural basis for phosphatidylinositol-phosphate biosynthesis.,Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F Nat Commun. 2015 Oct 16;6:8505. doi: 10.1038/ncomms9505. PMID:26510127<ref>PMID:26510127</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5d91" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Archaeoglobus fulgidus DSM 4304]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Kim | [[Category: Renibacterium salmoninarum ATCC 33209]] | ||
[[Category: Mancia | [[Category: Banerjee S]] | ||
[[Category: | [[Category: Belcher Dufrisne M]] | ||
[[Category: Clarke OB]] | |||
[[Category: Hendrickson WA]] | |||
[[Category: Jorge CD]] | |||
[[Category: Kim M]] | |||
[[Category: Mancia F]] | |||
[[Category: Rajashankar KR]] | |||
[[Category: Santos H]] | |||
[[Category: Tomasek DT]] | |||