5bvg: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5bvg is ON HOLD
+==Selenium incorporated nitrogenase MoFe-protein (Av1-Se2B) from A. vinelandii==
+<StructureSection load='5bvg' size='340' side='right'caption='[[5bvg]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5bvg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BVG FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CLF:FE(8)-S(7)+CLUSTER'>CLF</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HCA:3-HYDROXY-3-CARBOXY-ADIPIC+ACID'>HCA</scene>, <scene name='pdbligand=ICG:IRON-SULFUR-MOLYBDENUM+CLUSTER+WITH+INTERSTITIAL+CARBON+WITH+SELENIUM+INCORPORATED'>ICG</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SE:SELENIUM+ATOM'>SE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bvg OCA], [https://pdbe.org/5bvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bvg RCSB], [https://www.ebi.ac.uk/pdbsum/5bvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bvg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NIFD_AZOVI NIFD_AZOVI] This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Dinitrogen reduction in the biological nitrogen cycle is catalyzed by nitrogenase, a two-component metalloenzyme. Understanding of the transformation of the inert resting state of the active site FeMo-cofactor into an activated state capable of reducing dinitrogen remains elusive. Here we report the catalysis dependent, site-selective incorporation of selenium into the FeMo-cofactor from selenocyanate as a newly identified substrate and inhibitor. The 1.60 A resolution structure reveals selenium occupying the S2B site of FeMo-cofactor in the Azotobacter vinelandii MoFe-protein, a position that was recently identified as the CO-binding site. The Se2B-labeled enzyme retains substrate reduction activity and marks the starting point for a crystallographic pulse-chase experiment of the active site during turnover. Through a series of crystal structures obtained at resolutions of 1.32-1.66 A, including the CO-inhibited form of Av1-Se2B, the exchangeability of all three belt-sulfur sites is demonstrated, providing direct insights into unforeseen rearrangements of the metal center during catalysis.
-Authors: Spatzal, T., Perez, K.A., Howard, J.B., Rees, D.C.
+Catalysis-dependent selenium incorporation and migration in the nitrogenase active site iron-molybdenum cofactor.,Spatzal T, Perez KA, Howard JB, Rees DC Elife. 2015 Dec 16;4. pii: e11620. doi: 10.7554/eLife.11620. PMID:26673079<ref>PMID:26673079</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Perez, K.A]]
+<div class="pdbe-citations 5bvg" style="background-color:#fffaf0;"></div>
-[[Category: Howard, J.B]]
-[[Category: Rees, D.C]]
+==See Also==
-[[Category: Spatzal, T]]
+*[[Nitrogenase 3D structures|Nitrogenase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Azotobacter vinelandii]]
+[[Category: Large Structures]]
+[[Category: Howard JB]]
+[[Category: Perez KA]]
+[[Category: Rees DC]]
+[[Category: Spatzal T]]