4zqu: Difference between revisions
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<StructureSection load='4zqu' size='340' side='right'caption='[[4zqu]], [[Resolution|resolution]] 2.09Å' scene=''> | <StructureSection load='4zqu' size='340' side='right'caption='[[4zqu]], [[Resolution|resolution]] 2.09Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4zqu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZQU OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4zqu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Rubislaw_str._ATCC_10717 Salmonella enterica subsp. enterica serovar Rubislaw str. ATCC 10717] and [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis_YPIII Yersinia pseudotuberculosis YPIII]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZQU FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zqu OCA], [https://pdbe.org/4zqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zqu RCSB], [https://www.ebi.ac.uk/pdbsum/4zqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zqu ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/CDIA_YERPY CDIA_YERPY] Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. The C-terminal 123 residues (954-1077) has DNase activity in the presence of Zn(2+), converting supercoiled DNA into open-circular form. Toxic activity is neutralized by coexpression of the cognate immunity protein CdiI-YPIII, but not by non-cognate immunity proteins from other toxin-immunity modules. Expression of the DNase domain as a chimera allows bacteria to attack other non-immune bacteria which become filamentous and have lost DNA staining.<ref>PMID:26449640</ref> The CdiA protein is thought to be exported from the cell through the central lumen of CdiB, the other half of its two-partner system (TPS). The TPS domain probably remains associated with CdiB while the FHA-1 domain forms an extended filament with the receptor-binding domain (RBD) at its extremity; in the secretion arrested state the C-terminus of the RBD and YP domains form a hairpin-like structure as the FHA-2, PT and CT domains are periplasmic. The YP domain is probably responsible for this arrest at the point where it re-enters the host cell periplasm. Upon binding to a target cell outer membrane receptor a signal is transmitted to activate secretion. The filament elongates slightly, the rest of CdiA is secreted and the FHA-2 domain becomes stably associated with the target cell's outer membrane where it facilitates entry of the toxic CT domain into the target cell periplasm. From there the toxic CT domain is cleaved and gains access to the target cell cytoplasm via an inner membrane protein. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Salmonella enterica subsp. enterica serovar Rubislaw str. ATCC 10717]] | ||
[[Category: | [[Category: Yersinia pseudotuberculosis YPIII]] | ||
[[Category: | [[Category: Credali A]] | ||
[[Category: | [[Category: Goulding CW]] | ||
[[Category: | [[Category: Johnson PM]] | ||
[[Category: | [[Category: Morse RP]] | ||