4uc4: Difference between revisions

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-'''Unreleased structure'''
-The entry 4uc4 is ON HOLD  until Nov 01 2016
+==Crystal structure of hybrid tudor domain of human lysine demethylase KDM4B==
+<StructureSection load='4uc4' size='340' side='right'caption='[[4uc4]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4uc4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UC4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5612&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uc4 OCA], [https://pdbe.org/4uc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uc4 RCSB], [https://www.ebi.ac.uk/pdbsum/4uc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uc4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KDM4B_HUMAN KDM4B_HUMAN] Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.<ref>PMID:16603238</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The KDM4 histone demethylases are conserved epigenetic regulators linked to development, spermatogenesis and tumorigenesis. However, how the KDM4 family targets specific chromatin regions is largely unknown. Here, an extensive histone peptide microarray analysis uncovers trimethyl-lysine histone-binding preferences among the closely related KDM4 double tudor domains (DTDs). KDM4A/B DTDs bind strongly to H3K23me3, a poorly understood histone modification recently shown to be enriched in meiotic chromatin of ciliates and nematodes. The 2.28 A co-crystal structure of KDM4A-DTD in complex with H3K23me3 peptide reveals key intermolecular interactions for H3K23me3 recognition. Furthermore, analysis of the 2.56 A KDM4B-DTD crystal structure pinpoints the underlying residues required for exclusive H3K23me3 specificity, an interaction supported by in vivo co-localization of KDM4B and H3K23me3 at heterochromatin in mammalian meiotic and newly postmeiotic spermatocytes. In vitro demethylation assays suggest H3K23me3 binding by KDM4B stimulates H3K36 demethylation. Together, these results provide a possible mechanism whereby H3K23me3-binding by KDM4B directs localized H3K36 demethylation during meiosis and spermatogenesis.
-Authors: Wang, F., Su, Z., Denu, J.M., Phillips Jr., G.N.
+Reader domain specificity and lysine demethylase-4 family function.,Su Z, Wang F, Lee JH, Stephens KE, Papazyan R, Voronina E, Krautkramer KA, Raman A, Thorpe JJ, Boersma MD, Kuznetsov VI, Miller MD, Taverna SD, Phillips GN Jr, Denu JM Nat Commun. 2016 Nov 14;7:13387. doi: 10.1038/ncomms13387. PMID:27841353<ref>PMID:27841353</ref>
-Description: Crystal structure of hybrid tudor domain of human lysine demethylase KDM4B
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: G.N]]
+<div class="pdbe-citations 4uc4" style="background-color:#fffaf0;"></div>
-[[Category: Su, Z]]
-[[Category: Denu, J.M]]
+==See Also==
-[[Category: Wang, F]]
+*[[Jumonji domain-containing protein 3D structures|Jumonji domain-containing protein 3D structures]]
-[[Category: Phillips Jr]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Denu JM]]
+[[Category: Phillips Jr GN]]
+[[Category: Su Z]]
+[[Category: Wang F]]