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==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form (oxidized state) - 3==
==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 3==
<StructureSection load='4ttt' size='340' side='right' caption='[[4ttt]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
<StructureSection load='4ttt' size='340' side='right'caption='[[4ttt]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ttt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TTT FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ttt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TTT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=F4S:FE4-S3+CLUSTER'>F4S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4iub|4iub]], [[4iuc|4iuc]], [[4iud|4iud]], [[3rgw|3rgw]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=F4S:FE4-S3+CLUSTER'>F4S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogenase_(acceptor) Hydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.99.6 1.12.99.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ttt OCA], [https://pdbe.org/4ttt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ttt RCSB], [https://www.ebi.ac.uk/pdbsum/4ttt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ttt ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ttt OCA], [http://pdbe.org/4ttt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ttt RCSB], [http://www.ebi.ac.uk/pdbsum/4ttt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ttt ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. [[http://www.uniprot.org/uniprot/MBHS_CUPNH MBHS_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.  
[https://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
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</StructureSection>
</StructureSection>
[[Category: Kalms, J]]
[[Category: Cupriavidus necator H16]]
[[Category: Scheerer, P]]
[[Category: Large Structures]]
[[Category: Schmidt, A]]
[[Category: Kalms J]]
[[Category: Aerobic hydrogen bacteria]]
[[Category: Scheerer P]]
[[Category: Bimetallic]]
[[Category: Schmidt A]]
[[Category: Dehydrogenase]]
[[Category: Hydrogen catalysis]]
[[Category: Hydrogenase]]
[[Category: Knallgasbacteria]]
[[Category: Membrane]]
[[Category: Membrane-bound]]
[[Category: Metalloenzyme]]
[[Category: Metalloprotein catalytic center]]
[[Category: Ni-fe active site]]
[[Category: Nife]]
[[Category: Oxidized state]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase-oxidoreductase complex]]
[[Category: Oxygen-tolerant hydrogenase]]
[[Category: Proteobacteria]]
[[Category: T-cluster]]

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