5k2c: Difference between revisions
Jump to navigation
Jump to search
m Protected "5k2c" [edit=sysop:move=sysop] |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==1.9 angstrom A2a adenosine receptor structure with sulfur SAD phasing and phase extension using XFEL data== | ||
<StructureSection load='5k2c' size='340' side='right'caption='[[5k2c]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5k2c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K2C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k2c OCA], [https://pdbe.org/5k2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k2c RCSB], [https://www.ebi.ac.uk/pdbsum/5k2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k2c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Serial femtosecond crystallography (SFX) takes advantage of extremely bright and ultrashort pulses produced by x-ray free-electron lasers (XFELs), allowing for the collection of high-resolution diffraction intensities from micrometer-sized crystals at room temperature with minimal radiation damage, using the principle of "diffraction-before-destruction." However, de novo structure factor phase determination using XFELs has been difficult so far. We demonstrate the ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms, leading to a bias-free room temperature structure of the human A2A adenosine receptor at 1.9 A resolution. The advancement was made possible by recent improvements in SFX data analysis and the design of injectors and delivery media for streaming hydrated microcrystals. This general method should accelerate structural studies of novel difficult-to-crystallize macromolecules and their complexes. | |||
Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.,Batyuk A, Galli L, Ishchenko A, Han GW, Gati C, Popov PA, Lee MY, Stauch B, White TA, Barty A, Aquila A, Hunter MS, Liang M, Boutet S, Pu M, Liu ZJ, Nelson G, James D, Li C, Zhao Y, Spence JC, Liu W, Fromme P, Katritch V, Weierstall U, Stevens RC, Cherezov V Sci Adv. 2016 Sep 23;2(9):e1600292. eCollection 2016 Sep. PMID:27679816<ref>PMID:27679816</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5k2c" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Adenosine A2A receptor 3D structures|Adenosine A2A receptor 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Fromme | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Aquila A]] | ||
[[Category: | [[Category: Barty A]] | ||
[[Category: | [[Category: Batyuk A]] | ||
[[Category: | [[Category: Boutet S]] | ||
[[Category: Li | [[Category: Cherezov V]] | ||
[[Category: | [[Category: Fromme P]] | ||
[[Category: | [[Category: Galli L]] | ||
[[Category: Liu | [[Category: Gati C]] | ||
[[Category: | [[Category: Han GW]] | ||
[[Category: | [[Category: Hunter MS]] | ||
[[Category: | [[Category: Ishchenko A]] | ||
[[Category: | [[Category: James D]] | ||
[[Category: | [[Category: Katritch V]] | ||
[[Category: | [[Category: Lee M-Y]] | ||
[[Category: | [[Category: Li C]] | ||
[[Category: | [[Category: Liang M]] | ||
[[Category: Liu W]] | |||
[[Category: Liu Z-J]] | |||
[[Category: Nelson G]] | |||
[[Category: Popov P]] | |||
[[Category: Pu M]] | |||
[[Category: Spence JCH]] | |||
[[Category: Stauch B]] | |||
[[Category: Stevens RC]] | |||
[[Category: Weierstall U]] | |||
[[Category: White TA]] | |||
[[Category: Zhao Y]] | |||
Latest revision as of 22:19, 20 September 2023
1.9 angstrom A2a adenosine receptor structure with sulfur SAD phasing and phase extension using XFEL data1.9 angstrom A2a adenosine receptor structure with sulfur SAD phasing and phase extension using XFEL data
Structural highlights
FunctionAA2AR_HUMAN Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.C562_ECOLX Electron-transport protein of unknown function. Publication Abstract from PubMedSerial femtosecond crystallography (SFX) takes advantage of extremely bright and ultrashort pulses produced by x-ray free-electron lasers (XFELs), allowing for the collection of high-resolution diffraction intensities from micrometer-sized crystals at room temperature with minimal radiation damage, using the principle of "diffraction-before-destruction." However, de novo structure factor phase determination using XFELs has been difficult so far. We demonstrate the ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms, leading to a bias-free room temperature structure of the human A2A adenosine receptor at 1.9 A resolution. The advancement was made possible by recent improvements in SFX data analysis and the design of injectors and delivery media for streaming hydrated microcrystals. This general method should accelerate structural studies of novel difficult-to-crystallize macromolecules and their complexes. Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.,Batyuk A, Galli L, Ishchenko A, Han GW, Gati C, Popov PA, Lee MY, Stauch B, White TA, Barty A, Aquila A, Hunter MS, Liang M, Boutet S, Pu M, Liu ZJ, Nelson G, James D, Li C, Zhao Y, Spence JC, Liu W, Fromme P, Katritch V, Weierstall U, Stevens RC, Cherezov V Sci Adv. 2016 Sep 23;2(9):e1600292. eCollection 2016 Sep. PMID:27679816[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||