4s2s: Difference between revisions
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==Crystal Structure of Fab fragment of monoclonal antibody RoAb13== | ==Crystal Structure of Fab fragment of monoclonal antibody RoAb13== | ||
<StructureSection load='4s2s' size='340' side='right' caption='[[4s2s]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='4s2s' size='340' side='right'caption='[[4s2s]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4s2s]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4s2s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S2S FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s2s OCA], [https://pdbe.org/4s2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s2s RCSB], [https://www.ebi.ac.uk/pdbsum/4s2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s2s ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 4s2s" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4s2s" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Akthar | [[Category: Akthar S]] | ||
[[Category: Arnold | [[Category: Arnold J]] | ||
[[Category: Chain | [[Category: Chain B]] | ||
[[Category: Chayen | [[Category: Chayen NE]] | ||
[[Category: Govada | [[Category: Govada L]] | ||
[[Category: Ji | [[Category: Ji C]] | ||
[[Category: Lapp | [[Category: Lapp T]] | ||
[[Category: Naider | [[Category: Naider D]] | ||
[[Category: Noursadeghi | [[Category: Noursadeghi M]] | ||
[[Category: Saridakis | [[Category: Saridakis E]] | ||
[[Category: Zhang | [[Category: Zhang Y]] | ||
Latest revision as of 21:02, 20 September 2023
Crystal Structure of Fab fragment of monoclonal antibody RoAb13Crystal Structure of Fab fragment of monoclonal antibody RoAb13
Structural highlights
Publication Abstract from PubMedThe CCR5 receptor plays a role in several key physiological and pathological processes and is an important therapeutic target. Inhibition of the CCR5 axis by passive or active immunisation offers one very selective strategy for intervention. In this study we define a new linear epitope within the extracellular domain of CCR5 recognised by two independently produced monoclonal antibodies. A short peptide encoding the linear epitope can induce antibodies which recognise the intact receptor when administered colinear with a tetanus toxoid helper T cell epitope. The monoclonal antibody RoAb 13 is shown to bind to both cells and peptide with moderate to high affinity (6x10^8 and 1.2x107 M-1 respectively), and binding to the peptide is enhanced by sulfation of tyrosines at positions 10 and 14. RoAb13, which has previously been shown to block HIV infection, also blocks migration of monocytes in response to CCR5 binding chemokines and to inflammatory macrophage conditioned medium. A Fab fragment of RoAb13 has been crystallised and a structure of the antibody is reported to 2.1 angstrom resolution. A Linear Epitope in the N-Terminal Domain of CCR5 and Its Interaction with Antibody.,Chain B, Arnold J, Akthar S, Brandt M, Davis D, Noursadeghi M, Lapp T, Ji C, Sankuratri S, Zhang Y, Govada L, Saridakis E, Chayen N PLoS One. 2015 Jun 1;10(6):e0128381. doi: 10.1371/journal.pone.0128381., eCollection 2015. PMID:26030924[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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