4rns: Difference between revisions
New page: '''Unreleased structure''' The entry 4rns is ON HOLD until Paper Publication Authors: Hayes, R.P., Moural, T.W., Lewis, K.M., Onofrei, D., Xun, L., Kang, C. Description: PcpR inducer b... |
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==PcpR inducer binding domain (apo-form)== | |||
<StructureSection load='4rns' size='340' side='right'caption='[[4rns]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4rns]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingobium_chlorophenolicum Sphingobium chlorophenolicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rns OCA], [https://pdbe.org/4rns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rns RCSB], [https://www.ebi.ac.uk/pdbsum/4rns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rns ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PCPR_SPHCR PCPR_SPHCR] Transcriptional activator for the pcpA, pcpB and pcpE genes for pentachlorophenol (PCP) degradation. Essential for PCP degradation.<ref>PMID:12169590</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
PcpR is a LysR-type transcription factor from Sphingobium chlorophenolicum L-1 that is responsible for the activation of several genes involved in polychlorophenol degradation. PcpR responds to several polychlorophenols in vivo. Here, we report the crystal structures of the inducer-binding domain of PcpR in the apo-form and binary complexes with pentachlorophenol (PCP) and 2,4,6-trichlorophenol (2,4,6-TCP). Both X-ray crystal structures and isothermal titration calorimetry data indicated the association of two PCP molecules per PcpR, but only one 2,4,6-TCP molecule. The hydrophobic nature and hydrogen bonds of one binding cavity allowed the tight association of both PCP (Kd = 110 nM) and 2,4,6-TCP (Kd = 22.8 nM). However, the other cavity was unique to PCP with much weaker affinity (Kd = 70 muM) and thus its significance was not clear. Neither phenol nor benzoic acid displayed any significant affinity to PcpR, indicating a role of chlorine substitution in ligand specificity. When PcpR is compared with TcpR, a LysR-type regulator controlling the expression of 2,4,6-trichlorophenol degradation in Cupriavidus necator JMP134, most of the residues constituting the two inducer-binding cavities of PcpR are different, except for their general hydrophobic nature. The finding concurs that PcpR uses various polychlorophenols as long as it includes 2,4,6-trichlorophenol, as inducers; whereas TcpR is only responsive to 2,4,6-trichlorophenol. | |||
Structures of the Inducer-Binding Domain of Pentachlorophenol-Degrading Gene Regulator PcpR from Sphingobium chlorophenolicum.,Hayes RP, Moural TW, Lewis KM, Onofrei D, Xun L, Kang C Int J Mol Sci. 2014 Nov 12;15(11):20736-52. doi: 10.3390/ijms151120736. PMID:25397598<ref>PMID:25397598</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4rns" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sphingobium chlorophenolicum]] | |||
[[Category: Hayes RP]] | |||
[[Category: Kang C]] | |||
[[Category: Lewis KM]] | |||
[[Category: Moural TW]] | |||
[[Category: Onofrei D]] | |||
[[Category: Xun L]] | |||