4pu8: Difference between revisions
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==Shewanella oneidensis Toxin Antitoxin System Antitoxin Protein HipB Resolution 2.35== | ==Shewanella oneidensis Toxin Antitoxin System Antitoxin Protein HipB Resolution 2.35== | ||
<StructureSection load='4pu8' size='340' side='right' caption='[[4pu8]], [[Resolution|resolution]] 2.35Å' scene=''> | <StructureSection load='4pu8' size='340' side='right'caption='[[4pu8]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4pu8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU8 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4pu8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PU8 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu8 OCA], [https://pdbe.org/4pu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pu8 RCSB], [https://www.ebi.ac.uk/pdbsum/4pu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/HIPB_SHEON HIPB_SHEON] Antitoxin component of a type II toxin-antitoxin (TA) system. Neutralizes the toxic effect of cognate toxin HipA; overexpression in wild-type or a hipAB deletion temporarily inhibits cell growth. Binds operator DNA; in the ternary phosphoserine-HipA-HipB-DNA complex the DNA is bent about 125 degrees.<ref>PMID:25056321</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 4pu8" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4pu8" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Behiels | [[Category: Large Structures]] | ||
[[Category: Devreese | [[Category: Shewanella oneidensis MR-1]] | ||
[[Category: Elegheert | [[Category: Behiels E]] | ||
[[Category: Felix | [[Category: Devreese B]] | ||
[[Category: Savvides | [[Category: Elegheert J]] | ||
[[Category: Vergauwen | [[Category: Felix J]] | ||
[[Category: Wen | [[Category: Savvides S]] | ||
[[Category: Vergauwen B]] | |||
[[Category: Wen Y]] | |||
Latest revision as of 20:22, 20 September 2023
Shewanella oneidensis Toxin Antitoxin System Antitoxin Protein HipB Resolution 2.35Shewanella oneidensis Toxin Antitoxin System Antitoxin Protein HipB Resolution 2.35
Structural highlights
FunctionHIPB_SHEON Antitoxin component of a type II toxin-antitoxin (TA) system. Neutralizes the toxic effect of cognate toxin HipA; overexpression in wild-type or a hipAB deletion temporarily inhibits cell growth. Binds operator DNA; in the ternary phosphoserine-HipA-HipB-DNA complex the DNA is bent about 125 degrees.[1] Publication Abstract from PubMedNearly all bacteria exhibit a type of phenotypic growth described as persistence that is thought to underlie antibiotic tolerance and recalcitrant chronic infections. The chromosomally encoded high-persistence (Hip) toxin-antitoxin proteins HipASO and HipBSO from Shewanella oneidensis, a proteobacterium with unusual respiratory capacities, constitute a type II toxin-antitoxin protein module. Here we show that phosphorylated HipASO can engage in an unexpected ternary complex with HipBSO and double-stranded operator DNA that is distinct from the prototypical counterpart complex from Escherichia coli. The structure of HipBSO in complex with operator DNA reveals a flexible C-terminus that is sequestered by HipASO in the ternary complex, indicative of its role in binding HipASO to abolish its function in persistence. The structure of HipASO in complex with a non-hydrolyzable ATP analogue shows that HipASO autophosphorylation is coupled to an unusual conformational change of its phosphorylation loop. However, HipASO is unable to phosphorylate the translation factor Elongation factor Tu, contrary to previous reports, but in agreement with more recent findings. Our studies suggest that the phosphorylation state of HipA is an important factor in persistence and that the structural and mechanistic diversity of HipAB modules as regulatory factors in bacterial persistence is broader than previously thought. The bacterial antitoxin HipB establishes a ternary complex with operator DNA and phosphorylated toxin HipA to regulate bacterial persistence.,Wen Y, Behiels E, Felix J, Elegheert J, Vergauwen B, Devreese B, Savvides SN Nucleic Acids Res. 2014 Jul 23. pii: gku665. PMID:25056321[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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