4oo9: Difference between revisions

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 ==Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane domain in complex with the negative allosteric modulator mavoglurant==
-<StructureSection load='4oo9' size='340' side='right' caption='[[4oo9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='4oo9' size='340' side='right'caption='[[4oo9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4oo9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OO9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4oo9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OO9 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2U8:MAVOGLURANT'>2U8</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene><br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=YCM:S-(2-AMINO-2-OXOETHYL)-L-CYSTEINE'>YCM</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2U8:MAVOGLURANT'>2U8</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=YCM:S-(2-AMINO-2-OXOETHYL)-L-CYSTEINE'>YCM</scene></td></tr>
-<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oo9 OCA], [https://pdbe.org/4oo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oo9 RCSB], [https://www.ebi.ac.uk/pdbsum/4oo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oo9 ProSAT]</span></td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oo9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oo9 RCSB], [http://www.ebi.ac.uk/pdbsum/4oo9 PDBsum]</span></td></tr>
+</table>
-<table>
+== Function ==
+[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [https://www.uniprot.org/uniprot/GRM5_HUMAN GRM5_HUMAN] Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
-The effects of pH variation on ionic exchange and mechanical function were studied in the arterially perfused rat and rabbit septa. The pH and PCO2 of the control perfusate were 7.40 and 39 mmHg, respectively. In the rabbit septum a metabolic acidosis (pH equals 6.82, PCO2 equals 39 mmHg) caused a loss of 16% of control tension in 12 min. Na+ and K+ exchange were unaltered. A comparable respiratory acidosis (pH equals 6.81, PCO2 equals 159 mmHg) caused a 51% loss of tension in 2 min. Na+ exchange was unaltered but K+ efflux fell from 8.9 +/- 0.6 (mean +/- SE) to 4.9 +/- 0.3 mmol/kg dry wt per min (P less than 0.001, n equals 10). A net gain of K+ of 16.9 +/- 1.7 (n equals 14) mmol/kg dry wt occurred and was attributable to a delayed fall in K+ influx relative to efflux over 15 min. The net gain could not be mimicked by epinephrine administration or blocked by propranolol and was absent in the beating rat septum and the quiescent rabbit septum. These results suggest that the net uptake of K+, which appears to be dependent on a period of depolarization, and the changes of contractility are controlled by the H+ ion concentration at a cellular site whose exchange with the extracellular space is characterized by a considerable restriction of diffusion. Changes of contractility are not related to the net uptake of K+.
+Metabotropic glutamate receptors are class C G-protein-coupled receptors which respond to the neurotransmitter glutamate. Structural studies have been restricted to the amino-terminal extracellular domain, providing little understanding of the membrane-spanning signal transduction domain. Metabotropic glutamate receptor 5 is of considerable interest as a drug target in the treatment of fragile X syndrome, autism, depression, anxiety, addiction and movement disorders. Here we report the crystal structure of the transmembrane domain of the human receptor in complex with the negative allosteric modulator, mavoglurant. The structure provides detailed insight into the architecture of the transmembrane domain of class C receptors including the precise location of the allosteric binding site within the transmembrane domain and key micro-switches which regulate receptor signalling. This structure also provides a model for all class C G-protein-coupled receptors and may aid in the design of new small-molecule drugs for the treatment of brain disorders.
-Effect of pH on ionic exchange and function in rat and rabbit myocardium.,Poole-Wilson PA, Langer GA Am J Physiol. 1975 Sep;229(3):570-81. PMID:2014<ref>PMID:2014</ref>
+Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain.,Dore AS, Okrasa K, Patel JC, Serrano-Vega M, Bennett K, Cooke RM, Errey JC, Jazayeri A, Khan S, Tehan B, Weir M, Wiggin GR, Marshall FH Nature. 2014 Jul 31;511(7511):557-62. doi: 10.1038/nature13396. Epub 2014 Jul 6. PMID:25042998<ref>PMID:25042998</ref>
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4oo9" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Metabotropic glutamate receptor 3D structures|Metabotropic glutamate receptor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Lysozyme]]
+[[Category: Escherichia virus T4]]
-[[Category: Bennett, K.]]
+[[Category: Homo sapiens]]
-[[Category: Cooke, R M.]]
+[[Category: Large Structures]]
-[[Category: Dore, A S.]]
+[[Category: Bennett K]]
-[[Category: Errey, J C.]]
+[[Category: Cooke RM]]
-[[Category: Jazayeri, A.]]
+[[Category: Dore AS]]
-[[Category: Khan, S.]]
+[[Category: Errey JC]]
-[[Category: Marshall, F H.]]
+[[Category: Jazayeri A]]
-[[Category: Okrasa, K.]]
+[[Category: Khan S]]
-[[Category: Patel, J C.]]
+[[Category: Marshall FH]]
-[[Category: Serrano-Vega, M.]]
+[[Category: Okrasa K]]
-[[Category: Tehan, B.]]
+[[Category: Patel JC]]
-[[Category: Weir, M.]]
+[[Category: Serrano-Vega M]]
-[[Category: Wiggin, G R.]]
+[[Category: Tehan B]]
-[[Category: 7tm]]
+[[Category: Weir M]]
-[[Category: Cysteine-s-acetamide]]
+[[Category: Wiggin GR]]
-[[Category: G-protein]]
-[[Category: Membrane protein]]
-[[Category: Receptor]]