4mzp: Difference between revisions

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 ==MazF from S. aureus crystal form III, C2221, 2.7 A==
-<StructureSection load='4mzp' size='340' side='right' caption='[[4mzp]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='4mzp' size='340' side='right'caption='[[4mzp]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4mzp]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MZP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4mzp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_N315 Staphylococcus aureus subsp. aureus N315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MZP FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mzm|4mzm]], [[4mzt|4mzt]], [[2mf2|2mf2]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.702&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mzp RCSB], [http://www.ebi.ac.uk/pdbsum/4mzp PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzp OCA], [https://pdbe.org/4mzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mzp RCSB], [https://www.ebi.ac.uk/pdbsum/4mzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzp ProSAT]</span></td></tr>
-<table>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MAZF_STAAN MAZF_STAAN] Toxic component of a toxin-antitoxin (TA) module. Ribosome-independent, sequence-specific endoribonuclease that cleaves mRNA, thus inhibiting protein synthesis and inducing bacterial stasis. Cleavages occur preferentially in a U-rich region with a consensus sequence of 5'-[ACG]UU[ACG]-3' in single-stranded RNA (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
-mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute a unique toxin-antitoxin module under regulation of the sigB operon. A MazF-type mRNA interferase is combined with an antitoxin of unknown fold. Crystals of S. aureus MazF (SaMazF) were grown in space group P2(1)2(1)2(1). The crystals diffracted to 2.1 A resolution and are likely to contain two SaMazF dimers in the asymmetric unit.
+The Staphylococcus aureus genome contains three toxin-antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ensembles are found in three loop regions, two of which undergo motions that are of functional relevance. The same segments also show functionally relevant dynamics in the distantly related CcdB family despite divergence of function. NMR chemical shift mapping and analysis of residue conservation in the MazF family suggests a conserved mode for the inhibition of MazF by MazE.
-Crystallization of the Staphylococcus aureus MazF mRNA interferase.,Zorzini V, Haesaerts S, Donegan NP, Fu Z, Cheung AL, van Nuland NA, Loris R Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):386-9. doi:, 10.1107/S1744309111000571. Epub 2011 Feb 25. PMID:21393849<ref>PMID:21393849</ref>
+Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics.,Zorzini V, Buts L, Sleutel M, Garcia-Pino A, Talavera A, Haesaerts S, Greve HD, Cheung A, van Nuland NA, Loris R Nucleic Acids Res. 2014 Jun 1;42(10):6709-25. doi: 10.1093/nar/gku266. Epub 2014 , Apr 19. PMID:24748664<ref>PMID:24748664</ref>
-From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4mzp" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Cheung, A.]]
+[[Category: Large Structures]]
-[[Category: Loris, R.]]
+[[Category: Staphylococcus aureus subsp. aureus N315]]
-[[Category: Nuland, N A.J van.]]
+[[Category: Cheung A]]
-[[Category: Zorzini, V.]]
+[[Category: Loris R]]
-[[Category: Ccdb/mazf fold]]
+[[Category: Zorzini V]]
-[[Category: Hydrolase]]
+[[Category: Van Nuland NAJ]]
-[[Category: Maze]]
-[[Category: Mrna interferase]]
-[[Category: Ribonuclease]]