4mx3: Difference between revisions
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== | ==Crystal Structure of PKA RIalpha Homodimer== | ||
[[http://www.uniprot.org/uniprot/KAP0_BOVIN KAP0_BOVIN | <StructureSection load='4mx3' size='340' side='right'caption='[[4mx3]], [[Resolution|resolution]] 3.88Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4mx3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MX3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.88Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx3 OCA], [https://pdbe.org/4mx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4mx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mx3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/KAP0_BOVIN KAP0_BOVIN] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The regulatory (R) subunit is the cAMP receptor of protein kinase A. Following cAMP binding, the inactive PKA holoenzyme complex separates into two active catalytic (C) subunits and a cAMP-bound R dimer. Thus far, only monomeric R structures have been solved, which fell short in explaining differences of cAMP binding for the full-length protein as compared to the truncated R subunits. Here we solved a full-length R-dimer structure that reflects the biologically relevant conformation, and this structure agrees well with small angle X-ray scattering. An isoform-specific interface is revealed between the protomers. This interface acts as an intermolecular sensor for cAMP and explains the cooperative character of cAMP binding to the RIalpha dimer. Mutagenesis of residues on this interface not only leads to structural and biochemical changes, but is also linked to Carney complex disease. | |||
PKA RIalpha Homodimer Structure Reveals an Intermolecular Interface with Implications for Cooperative cAMP Binding and Carney Complex Disease.,Bruystens JG, Wu J, Fortezzo A, Kornev AP, Blumenthal DK, Taylor SS Structure. 2014 Jan 7;22(1):59-69. doi: 10.1016/j.str.2013.10.012. Epub 2013 Dec , 5. PMID:24316401<ref>PMID:24316401</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
< | </div> | ||
[[ | <div class="pdbe-citations 4mx3" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
[[Category: | *[[CAMP-dependent protein kinase 3D structures|CAMP-dependent protein kinase 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Bos taurus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Blumenthal DA]] | ||
[[Category: Bruystens JGH]] | |||
[[Category: Fortezzo A]] | |||
[[Category: Kornev AP]] | |||
[[Category: Taylor SS]] | |||
[[Category: Wu J]] | |||