4maf: Difference between revisions

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-{{STRUCTURE_4maf|  PDB=4maf  |  SCENE=  }}
-===Soybean ATP Sulfurylase===
-{{ABSTRACT_PUBMED_24584934}}
-==About this Structure==
+==Soybean ATP Sulfurylase==
-[[4maf]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAF OCA].
+<StructureSection load='4maf' size='340' side='right'caption='[[4maf]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4maf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MAF FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.48&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4maf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4maf OCA], [https://pdbe.org/4maf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4maf RCSB], [https://www.ebi.ac.uk/pdbsum/4maf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4maf ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8SAG1_SOYBN Q8SAG1_SOYBN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Enzymes of the sulfur assimilation pathway are potential targets for improving nutrient content and environmental stress responses in plants. The committed step in this pathway is catalyzed by ATP sulfurylase, which synthesizes adenosine-5'-phosphosulfate (APS) from sulfate and ATP. To better understand the molecular basis of this energetically unfavorable reaction, the x-ray crystal structure of ATP sulfurylase isoform 1 from soybean (GmATPS) in complex with APS was determined. This structure revealed several highly conserved substrate-binding motifs in the active site and a distinct dimerization interface compared to other ATP sulfurylases, but similar to mammalian PAPS synthetase. Steady-state kinetic analysis of twenty GmATPS point mutants suggests a reaction mechanism in which nucleophilic attack by sulfate on the alpha-phosphate of ATP involves transition state stabilization by Arg248, Asn249, His255, and Arg349. The structure and kinetic analysis suggest that ATP sulfurylase overcomes the energetic barrier of APS synthesis by distorting nucleotide structure and identifies critical residues for catalysis. Mutations that alter sulfate assimilation in Arabidopsis were mapped to the structure, which provides a molecular basis for understanding their effects on the sulfur assimilation pathway.
-==Reference==
+Structure and Mechanism of Soybean ATP Sulfurylase and the Committed Step in Plant Sulfur Assimilation.,Herrmann J, Ravilious GE, McKinney SE, Westfall CS, Lee SG, Baraniecka P, Giovannetti M, Kopriva S, Krishnan HB, Jez JM J Biol Chem. 2014 Feb 28. PMID:24584934<ref>PMID:24584934</ref>
-<ref group="xtra">PMID:024584934</ref><references group="xtra"/><references/>
-[[Category: Sulfate adenylyltransferase]]
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Herrmann, J.]]
+</div>
-[[Category: Jez, J M.]]
+<div class="pdbe-citations 4maf" style="background-color:#fffaf0;"></div>
-[[Category: Krishnan, H B.]]
+== References ==
-[[Category: Lee, S G.]]
+<references/>
-[[Category: McKinney, S E.]]
+__TOC__
-[[Category: Ravilious, G E.]]
+</StructureSection>
-[[Category: Westfall, C S.]]
+[[Category: Glycine max]]
-[[Category: Atp sulfurylase]]
+[[Category: Large Structures]]
-[[Category: Sulfur metabolism]]
+[[Category: Herrmann J]]
-[[Category: Transferase]]
+[[Category: Jez JM]]
+[[Category: Krishnan HB]]
+[[Category: Lee SG]]
+[[Category: McKinney SE]]
+[[Category: Ravilious GE]]
+[[Category: Westfall CS]]