4m2q: Difference between revisions

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-{{STRUCTURE_4m2q|  PDB=4m2q  |  SCENE=  }}
-===Crystal structure of non-myristoylated recoverin with Cysteine-39 oxidized to sulfenic acid===
-==Function==
+==Crystal structure of non-myristoylated recoverin with Cysteine-39 oxidized to sulfenic acid==
-[[http://www.uniprot.org/uniprot/RECO_BOVIN RECO_BOVIN]] Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.<ref>PMID:8097896</ref> <ref>PMID:8392055</ref>
+<StructureSection load='4m2q' size='340' side='right'caption='[[4m2q]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4m2q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M2Q FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m2q OCA], [https://pdbe.org/4m2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m2q RCSB], [https://www.ebi.ac.uk/pdbsum/4m2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m2q ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RECO_BOVIN RECO_BOVIN] Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.<ref>PMID:8097896</ref> <ref>PMID:8392055</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Recoverin, a 23-kDa Ca(2+)-binding protein of the neuronal calcium sensing (NCS) family, inhibits rhodopsin kinase, a Ser/Thr kinase responsible for termination of photoactivated rhodopsin in rod photoreceptor cells. Recoverin has two functional EF hands and a myristoylated N terminus. The myristoyl chain imparts cooperativity to the Ca(2+)-binding sites through an allosteric mechanism involving a conformational equilibrium between R and T states of the protein. Ca(2+) binds preferentially to the R state; the myristoyl chain binds preferentially to the T state. In the absence of myristoylation, the R state predominates, and consequently, binding of Ca(2+) to the non-myristoylated protein is not cooperative. We show here that a mutation, C39A, of a highly conserved Cys residue among NCS proteins, increases the apparent cooperativity for binding of Ca(2+) to non-myristoylated recoverin. The binding data can be explained by an effect on the T/R equilibrium to favor the T state without affecting the intrinsic binding constants for the two Ca(2+) sites.
-==About this Structure==
+A Highly Conserved Cysteine of Neuronal Calcium-sensing Proteins Controls Cooperative Binding of Ca2+ to Recoverin.,Ranaghan MJ, Kumar RP, Chakrabarti KS, Buosi V, Kern D, Oprian DD J Biol Chem. 2013 Dec 13;288(50):36160-7. doi: 10.1074/jbc.M113.524355. Epub 2013, Nov 4. PMID:24189072<ref>PMID:24189072</ref>
-[[4m2q]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M2Q OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<references group="xtra"/><references/>
+</div>
-[[Category: Chakrabarti, K.]]
+<div class="pdbe-citations 4m2q" style="background-color:#fffaf0;"></div>
-[[Category: Kern, D.]]
-[[Category: Kumar, R Prem.]]
+==See Also==
-[[Category: Oprian, D D.]]
+*[[Recoverin%2C a calcium-activated myristoyl switch|Recoverin%2C a calcium-activated myristoyl switch]]
-[[Category: Calcium binding protein]]
+== References ==
-[[Category: Ef hand]]
+<references/>
-[[Category: Inhibits rhodopsin kinase]]
+__TOC__
-[[Category: Metal binding protein]]
+</StructureSection>
-[[Category: Retina]]
+[[Category: Bos taurus]]
-[[Category: Rhodopsin kinase]]
+[[Category: Large Structures]]
+[[Category: Chakrabarti K]]
+[[Category: Kern D]]
+[[Category: Oprian DD]]
+[[Category: Prem Kumar R]]