4lw5: Difference between revisions

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-{{STRUCTURE_4lw5|  PDB=4lw5  |  SCENE=  }}
-===Crystal structure of all-trans green fluorescent protein===
-{{ABSTRACT_PUBMED_24408076}}
-==Function==
+==Crystal structure of all-trans green fluorescent protein==
-[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+<StructureSection load='4lw5' size='340' side='right'caption='[[4lw5]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4lw5]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LW5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lw5 OCA], [https://pdbe.org/4lw5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lw5 RCSB], [https://www.ebi.ac.uk/pdbsum/4lw5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lw5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Wild-type green fluorescent protein (GFP) folds on a time scale of minutes. The slow step in folding is a cis-trans peptide bond isomerization. The only conserved cis-peptide bond in the native GFP structure, at P89, was remodeled by the insertion of two residues, followed by iterative energy minimization and side chain design. The engineered GFP was synthesized and found to fold faster and more efficiently than its template protein, recovering 50% more of its fluorescence upon refolding. The slow phase of folding is faster and smaller in amplitude, and hysteresis in refolding has been eliminated. The elimination of a previously reported kinetically trapped state in refolding suggests that X-P89 is trans in the trapped state. A 2.55 A resolution crystal structure revealed that the new variant contains only trans-peptide bonds, as designed. This is the first instance of a computationally remodeled fluorescent protein that folds faster and more efficiently than wild type.
-==About this Structure==
+Green-lighting green fluorescent protein: Faster and more efficient folding by eliminating a cis-trans peptide isomerization event.,Rosenman DJ, Huang YM, Xia K, Fraser K, Jones VE, Lamberson CM, Van Roey P, Colon W, Bystroff C Protein Sci. 2014 Jan 9. doi: 10.1002/pro.2421. PMID:24408076<ref>PMID:24408076</ref>
-[[4lw5]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW5 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024408076</ref><references group="xtra"/><references/>
+</div>
-[[Category: Bystroff, C.]]
+<div class="pdbe-citations 4lw5" style="background-color:#fffaf0;"></div>
-[[Category: Colon, W.]]
-[[Category: Huang, Y M.]]
+==See Also==
-[[Category: Rosenman, D J.]]
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-[[Category: Vanroey, P.]]
+== References ==
-[[Category: Xia, K.]]
+<references/>
-[[Category: 11-stranded beta barrel]]
+__TOC__
-[[Category: Fluorescent protein]]
+</StructureSection>
+[[Category: Aequorea victoria]]
+[[Category: Large Structures]]
+[[Category: Bystroff C]]
+[[Category: Colon W]]
+[[Category: Huang Y-M]]
+[[Category: Rosenman DJ]]
+[[Category: Vanroey P]]
+[[Category: Xia K]]