4lvc: Difference between revisions

<StructureSection load='4lvc' size='340' side='right'caption='[[4lvc]], [[Resolution|resolution]] 1.74&Aring;' scene=''>

<table><tr><td colspan='2'>[[4lvc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_elkanii Bradyrhizobium elkanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LVC FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvc OCA], [https://pdbe.org/4lvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lvc RCSB], [https://www.ebi.ac.uk/pdbsum/4lvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvc ProSAT]</span></td></tr>

S-Adenosyl-L-homocysteine hydrolase (SAHase) is involved in the enzymatic regulation of S-adenosyl-L-methionine (SAM)-dependent methylation reactions. After methyl-group transfer from SAM, S-adenosyl-L-homocysteine (SAH) is formed as a byproduct, which in turn is hydrolyzed to adenosine (Ado) and homocysteine (Hcy) by SAHase. The crystal structure of BeSAHase, an SAHase from Bradyrhizobium elkanii, which is a nitrogen-fixing bacterial symbiont of legume plants, was determined at 1.7 A resolution, showing the domain organization (substrate-binding domain, NAD(+) cofactor-binding domain and dimerization domain) of the subunits. The protein crystallized in its biologically relevant tetrameric form, with three subunits in a closed conformation enforced by complex formation with the Ado product of the enzymatic reaction. The fourth subunit is ligand-free and has an open conformation. The BeSAHase structure therefore provides a unique snapshot of the domain movement of the enzyme induced by the binding of its natural ligands.

An enzyme captured in two conformational states: crystal structure of S-adenosyl-L-homocysteine hydrolase from Bradyrhizobium elkanii.,Manszewski T, Singh K, Imiolczyk B, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2422-32. doi:, 10.1107/S1399004715018659. Epub 2015 Nov 26. PMID:26627650<ref>PMID:26627650</ref>

[[Category: Bradyrhizobium elkanii]]

[[Category: Large Structures]]

[[Category: Imiolczyk B]]

[[Category: Jaskolski M]]

[[Category: Manszewski T]]

[[Category: Singh K]]