4led: Difference between revisions
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==The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms== | ==The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms== | ||
<StructureSection load='4led' size='340' side='right' caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> | <StructureSection load='4led' size='340' side='right'caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4led]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4led]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_str._C_1433 Pseudomonas aeruginosa str. C 1433]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LED FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4led FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4led OCA], [https://pdbe.org/4led PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4led RCSB], [https://www.ebi.ac.uk/pdbsum/4led PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4led ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/T2LG16_PSEAI T2LG16_PSEAI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Pseudomonas aeruginosa str. | [[Category: Large Structures]] | ||
[[Category: Cogdell | [[Category: Pseudomonas aeruginosa str. C 1433]] | ||
[[Category: Grinter | [[Category: Cogdell CJ]] | ||
[[Category: Mccaughey | [[Category: Grinter R]] | ||
[[Category: Roszak | [[Category: Mccaughey L]] | ||
[[Category: Walker | [[Category: Roszak AW]] | ||
[[Category: Walker D]] | |||
Latest revision as of 19:18, 20 September 2023
The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 AngstromsThe Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms
Structural highlights
FunctionPublication Abstract from PubMedLectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of d-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing d-rhamnose and not d-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of d-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins. Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor.,McCaughey LC, Grinter R, Josts I, Roszak AW, Waloen KI, Cogdell RJ, Milner J, Evans T, Kelly S, Tucker NP, Byron O, Smith B, Walker D PLoS Pathog. 2014 Feb 6;10(2):e1003898. doi: 10.1371/journal.ppat.1003898., eCollection 2014 Feb. PMID:24516380[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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