4jx1: Difference between revisions

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-{{STRUCTURE_4jx1|  PDB=4jx1  |  SCENE=  }}
-===Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor===
-{{ABSTRACT_PUBMED_23744947}}
-==Function==
+==Crystal structure of reduced Cytochrome P450cam-putidaredoxin complex bound to camphor and 5-exo-hydroxycamphor==
-[[http://www.uniprot.org/uniprot/CPXA_PSEPU CPXA_PSEPU]] Involved in a camphor oxidation system. [[http://www.uniprot.org/uniprot/PUTX_PSEPU PUTX_PSEPU]] The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.
+<StructureSection load='4jx1' size='340' side='right'caption='[[4jx1]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4jx1]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JX1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.087&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1N0:1,1-HEXANE-1,6-DIYLDIPYRROLIDINE-2,5-DIONE'>1N0</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CAH:5-EXO-HYDROXYCAMPHOR'>CAH</scene>, <scene name='pdbligand=CAM:CAMPHOR'>CAM</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jx1 OCA], [https://pdbe.org/4jx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4jx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jx1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CPXA_PSEPU CPXA_PSEPU] Involved in a camphor oxidation system.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cytochromes P450 catalyze a variety of monooxygenase reactions that require electron transfer from redox partners. Although the structure of many P450s and a small handful of redox partners are known, there is very little structural information available on redox complexes, thus leaving a gap in our understanding on the control of P450-redox partner interactions. We have solved the crystal structure of oxidized and reduced P450cam complexed with its redox partner, putidaredoxin (Pdx), to 2.2 and 2.09 angstroms, respectively. It was anticipated that Pdx would favor closed substrate-bound P450cam, which differs substantially from the open conformer, but instead we found that Pdx favors the open state. These new structures indicate that the effector role of Pdx is to shift P450cam toward the open conformation, which enables the establishment of a water-mediated H-bonded network, which is required for proton-coupled electron transfer.
-==About this Structure==
+Structural basis for effector control and redox partner recognition in cytochrome P450.,Tripathi S, Li H, Poulos TL Science. 2013 Jun 7;340(6137):1227-30. doi: 10.1126/science.1235797. PMID:23744947<ref>PMID:23744947</ref>
-[[4jx1]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JX1 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:023744947</ref><references group="xtra"/><references/>
+</div>
-[[Category: Camphor 5-monooxygenase]]
+<div class="pdbe-citations 4jx1" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
+*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Pseudomonas putida]]
-[[Category: Li, H.]]
+[[Category: Li H]]
-[[Category: Poulos, T L.]]
+[[Category: Poulos TL]]
-[[Category: Tripathi, S M.]]
+[[Category: Tripathi SM]]
-[[Category: Oxidoreductase-electron transport complex]]
-[[Category: Redox partner]]
-[[Category: Reduced p450cam-pdx complex]]