4j6x: Difference between revisions
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== | ==Crystal structure of Hfq from Pseudomonas aeruginosa in complex with UTP== | ||
[[http://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE | <StructureSection load='4j6x' size='340' side='right'caption='[[4j6x]], [[Resolution|resolution]] 2.22Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4j6x]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J6X FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.222Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j6x OCA], [https://pdbe.org/4j6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j6x RCSB], [https://www.ebi.ac.uk/pdbsum/4j6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j6x ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface. | |||
Hfq binds ribonucleotides in three different RNA-binding sites.,Murina V, Lekontseva N, Nikulin A Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473<ref>PMID:23897473</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
< | </div> | ||
[[ | <div class="pdbe-citations 4j6x" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Protein Hfq 3D structures|Protein Hfq 3D structures]] | |||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Pseudomonas aeruginosa PAO1]] | |||
[[Category: Lekontseva N]] | |||
[[Category: Murina V]] | |||
[[Category: Nikulin AD]] | |||