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==Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa T95A mutant==
==Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa T95A mutant==
<StructureSection load='4j5g' size='340' side='right' caption='[[4j5g]], [[Resolution|resolution]] 1.31&Aring;' scene=''>
<StructureSection load='4j5g' size='340' side='right'caption='[[4j5g]], [[Resolution|resolution]] 1.31&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4j5g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5G FirstGlance]. <br>
<table><tr><td colspan='2'>[[4j5g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J5G FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.31&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1sar|1sar]], [[1lni|1lni]], [[1gmp|1gmp]], [[1axy|1axy]], [[1t2i|1t2i]], [[1t2h|1t2h]], [[1i70|1i70]], [[1zgx|1zgx]], [[1i8v|1i8v]], [[1box|1box]], [[2sar|2sar]], [[4j5k|4j5k]], [[4gho|4gho]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5g OCA], [https://pdbe.org/4j5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j5g RCSB], [https://www.ebi.ac.uk/pdbsum/4j5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5g ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5g RCSB], [http://www.ebi.ac.uk/pdbsum/4j5g PDBsum]</span></td></tr>
</table>
<table>
== Function ==
[https://www.uniprot.org/uniprot/RNSA_KITAU RNSA_KITAU]
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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Contribution of hydrogen bonds to protein stability.,Pace CN, Fu H, Lee Fryar K, Landua J, Trevino SR, Schell D, Thurlkill RL, Imura S, Scholtz JM, Gajiwala K, Sevcik J, Urbanikova L, Myers JK, Takano K, Hebert EJ, Shirley BA, Grimsley GR Protein Sci. 2014 May;23(5):652-61. doi: 10.1002/pro.2449. Epub 2014 Mar 25. PMID:24591301<ref>PMID:24591301</ref>
Contribution of hydrogen bonds to protein stability.,Pace CN, Fu H, Lee Fryar K, Landua J, Trevino SR, Schell D, Thurlkill RL, Imura S, Scholtz JM, Gajiwala K, Sevcik J, Urbanikova L, Myers JK, Takano K, Hebert EJ, Shirley BA, Grimsley GR Protein Sci. 2014 May;23(5):652-61. doi: 10.1002/pro.2449. Epub 2014 Mar 25. PMID:24591301<ref>PMID:24591301</ref>


From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 4j5g" style="background-color:#fffaf0;"></div>
==See Also==
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Sevcik, J.]]
[[Category: Kitasatospora aureofaciens]]
[[Category: Urbanikova, L.]]
[[Category: Large Structures]]
[[Category: Endoribonuclease]]
[[Category: Sevcik J]]
[[Category: Hydrolase]]
[[Category: Urbanikova L]]
[[Category: Mutant]]

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