4iot: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:4iot.jpg|left|200px]]
-{{STRUCTURE_4iot|  PDB=4iot  |  SCENE=  }}
+==High-resolution Structure of Triosephosphate isomerase from E. coli==
+<StructureSection load='4iot' size='340' side='right'caption='[[4iot]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4iot]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._DH10B Escherichia coli str. K-12 substr. DH10B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IOT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iot OCA], [https://pdbe.org/4iot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iot RCSB], [https://www.ebi.ac.uk/pdbsum/4iot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iot ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TPIS_ECODH TPIS_ECODH]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Attempts to crystallize several mammalian proteins overexpressed in Escherichia coli revealed a common contaminant, triosephosphate isomerase, a protein involved in glucose metabolism. Even with triosephosphate isomerase present in very small amounts, similarly shaped crystals appeared in the crystallization drops in a number of polyethylene glycol-containing conditions. All of the target proteins were His-tagged and their purification involved immobilized metal-affinity chromatography (IMAC), a step that was likely to lead to triosephosphate isomerase contamination. Analysis of the triosephosphate isomerase crystals led to the structure of E. coli triosephosphate isomerase at 1.85 A resolution, which is a significant improvement over the previous structure.
-===High-resolution Structure of Triosephosphate isomerase from E. coli===
+Triosephosphate isomerase is a common crystallization contaminant of soluble His-tagged proteins produced in Escherichia coli.,Kozlov G, Vinaik R, Gehring K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May;69(Pt 5):499-502. doi:, 10.1107/S1744309113010841. Epub 2013 Apr 30. PMID:23695562<ref>PMID:23695562</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4iot" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4iot]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOT OCA].
+*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
-[[Category: Escherichia coli]]
+== References ==
-[[Category: Triose-phosphate isomerase]]
+<references/>
-[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
+__TOC__
-[[Category: Gehring, K.]]
+</StructureSection>
-[[Category: Kozlov, G.]]
+[[Category: Escherichia coli str. K-12 substr. DH10B]]
-[[Category: Vinaik, R.]]
+[[Category: Large Structures]]
-[[Category: Bsgi]]
+[[Category: Gehring K]]
-[[Category: Conversion of dihydroxyacetone phosphate to d-glyceraldehyde-3-phosphate]]
+[[Category: Kozlov G]]
-[[Category: Cytosol]]
+[[Category: Vinaik R]]
-[[Category: Isomerase]]
-[[Category: Montreal-kingston bacterial structural genomics initiative]]
-[[Category: Structural genomic]]
-[[Category: Tim barrel]]