4ilq: Difference between revisions
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The | ==2.60A resolution structure of CT771 from Chlamydia trachomatis== | ||
<StructureSection load='4ilq' size='340' side='right'caption='[[4ilq]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ilq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis_434/Bu Chlamydia trachomatis 434/Bu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILQ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ilq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilq OCA], [https://pdbe.org/4ilq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ilq RCSB], [https://www.ebi.ac.uk/pdbsum/4ilq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ilq ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0H3MCJ9_CHLT2 A0A0H3MCJ9_CHLT2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Asymmetric diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap4A) hydrolases are members of the Nudix superfamily that asymmetrically cleave the metabolite Ap4A into ATP and AMP while facilitating homeostasis. The obligate intracellular mammalian pathogen Chlamydia trachomatis possesses a single Nudix family protein, CT771. As pathogens that rely on a host for replication and dissemination typically have one or zero Nudix family proteins, this suggests that CT771 could be critical for chlamydial biology and pathogenesis. We identified orthologues to CT771 within environmental Chlamydiales that share active site residues suggesting a common function. Crystal structures of both apo- and ligand-bound CT771 were determined to 2.6 A and 1.9 A resolution, respectively. The structure of CT771 shows a alphabetaalpha-sandwich motif with many conserved elements lining the putative Nudix active site. Numerous aspects of the ligand-bound CT771 structure mirror those observed in the ligand-bound structure of the Ap4A hydrolase from Caenorhabditis elegans. These structures represent only the second Ap4A hydrolase enzyme member determined from eubacteria and suggest that mammalian and bacterial Ap4A hydrolases might be more similar than previously thought. The aforementioned structural similarities, in tandem with molecular docking, guided the enzymatic characterization of CT771. Together, these studies provide the molecular details for substrate binding and specificity, supporting the analysis that CT771 is an Ap4A hydrolase (nudH). | |||
Chlamydia trachomatis CT771 (nudH) Is an Asymmetric Ap4A Hydrolase.,Barta ML, Lovell S, Sinclair AN, Battaile KP, Hefty PS Biochemistry. 2014 Jan 14;53(1):214-24. doi: 10.1021/bi401473e. Epub 2013 Dec 31. PMID:24354275<ref>PMID:24354275</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4ilq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chlamydia trachomatis 434/Bu]] | |||
[[Category: Large Structures]] | |||
[[Category: Barta ML]] | |||
[[Category: Battaile KP]] | |||
[[Category: Hefty PS]] | |||
[[Category: Lovell S]] | |||