2frp: Difference between revisions

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-[[Image:2frp.png|left|200px]]
-{{STRUCTURE_2frp|  PDB=2frp  |  SCENE=  }}
+==Bacteriophage HK97 Expansion Intermediate IV==
+<SX load='2frp' size='340' side='right' viewer='molstar' caption='[[2frp]], [[Resolution|resolution]] 7.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2frp]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FRP FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2frp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2frp OCA], [https://pdbe.org/2frp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2frp RCSB], [https://www.ebi.ac.uk/pdbsum/2frp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2frp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/2frp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2frp ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
-===Bacteriophage HK97 Expansion Intermediate IV===
+Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM.,Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE Structure. 2006 Nov;14(11):1655-65. PMID:17098191<ref>PMID:17098191</ref>
-{{ABSTRACT_PUBMED_17098191}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2frp" style="background-color:#fffaf0;"></div>
-[[2frp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk97 Enterobacteria phage hk97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FRP OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:017098191</ref><references group="xtra"/>
+</SX>
-[[Category: Enterobacteria phage hk97]]
+[[Category: Escherichia virus HK97]]
-[[Category: Cheng, N.]]
+[[Category: Large Structures]]
-[[Category: Conway, J F.]]
+[[Category: Cheng N]]
-[[Category: Duda, R L.]]
+[[Category: Conway JF]]
-[[Category: Firek, B A.]]
+[[Category: Duda RL]]
-[[Category: Gan, L.]]
+[[Category: Firek BA]]
-[[Category: Hendrix, R W.]]
+[[Category: Gan L]]
-[[Category: Johnson, J E.]]
+[[Category: Hendrix RW]]
-[[Category: Lander, G.]]
+[[Category: Johnson JE]]
-[[Category: Liljas, L.]]
+[[Category: Lander G]]
-[[Category: Speir, J A.]]
+[[Category: Liljas L]]
-[[Category: Bacteriophage]]
+[[Category: Speir JA]]
-[[Category: Capsid protein]]
-[[Category: Expansion intermediate]]
-[[Category: Hk97]]
-[[Category: Icosahedral virus]]
-[[Category: Virus]]