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[[Image:1b7t.gif|left|200px]]
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{{STRUCTURE_1b7t|  PDB=1b7t  |  SCENE=  }}
'''MYOSIN DIGESTED BY PAPAIN'''


==MYOSIN DIGESTED BY PAPAIN==
<StructureSection load='1b7t' size='340' side='right'caption='[[1b7t]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1b7t]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. The June 2001 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Myosin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2001_6 10.2210/rcsb_pdb/mom_2001_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B7T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7t OCA], [https://pdbe.org/1b7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b7t RCSB], [https://www.ebi.ac.uk/pdbsum/1b7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MYS_ARGIR MYS_ARGIR] Muscle contraction.  Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b7t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b7t ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin.


==Overview==
Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head.,Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG, Cohen C Cell. 1999 May 14;97(4):459-70. PMID:10338210<ref>PMID:10338210</ref>
The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1B7T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. The following page contains interesting information on the relation of 1B7T with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb18_1.html Myosin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7T OCA].
</div>
<div class="pdbe-citations 1b7t" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head., Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG, Cohen C, Cell. 1999 May 14;97(4):459-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10338210 10338210]
*[[Myosin 3D Structures|Myosin 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Argopecten irradians]]
[[Category: Argopecten irradians]]
[[Category: Large Structures]]
[[Category: Myosin]]
[[Category: Myosin]]
[[Category: Protein complex]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Cohen, C.]]
[[Category: Cohen C]]
[[Category: Himmel, D.]]
[[Category: Himmel D]]
[[Category: Houdusse, A.]]
[[Category: Houdusse A]]
[[Category: Kalabokis, V.]]
[[Category: Kalabokis V]]
[[Category: Szent-Gyorgyi, A G.]]
[[Category: Szent-Gyorgyi AG]]
[[Category: Myosin motor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:10:58 2008''

Latest revision as of 17:49, 20 September 2023

MYOSIN DIGESTED BY PAPAINMYOSIN DIGESTED BY PAPAIN

Structural highlights

1b7t is a 3 chain structure with sequence from Argopecten irradians. The June 2001 RCSB PDB Molecule of the Month feature on Myosin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2001_6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYS_ARGIR Muscle contraction. Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin.

Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head.,Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG, Cohen C Cell. 1999 May 14;97(4):459-70. PMID:10338210[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG, Cohen C. Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head. Cell. 1999 May 14;97(4):459-70. PMID:10338210

1b7t, resolution 2.50Å

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