3pq6: Difference between revisions

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-[[Image:3pq6.png|left|200px]]
-<!--
+==Structure of I274C variant of E. coli KatE[] - Images 25-30==
-The line below this paragraph, containing "STRUCTURE_3pq6", creates the "Structure Box" on the page.
+<StructureSection load='3pq6' size='340' side='right'caption='[[3pq6]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3pq6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PQ6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene></td></tr>
-{{STRUCTURE_3pq6|  PDB=3pq6  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pq6 OCA], [https://pdbe.org/3pq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pq6 RCSB], [https://www.ebi.ac.uk/pdbsum/3pq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pq6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Heme-containing catalases have been extensively studied, revealing the roles of many residues, the existence of two heme orientations, flipped 180 degrees relative to one another along the propionate-vinyl axis, and the presence of both heme b and heme d. The focus of this report is a residue, situated adjacent to the vinyl groups of the heme at the entrance of the lateral channel, with an unusual main chain geometry that is conserved in all catalase structures so far determined. In Escherichia coli catalase HPII, the residue is Ile274, and replacing it with Gly, Ala, and Val, found at the same location in other catalases, results in a reduction in catalytic efficiency, a reduced intensity of the Soret absorbance band, and a mixture of heme orientations and species. The reduced turnover rates and higher H(2)O(2) concentrations required to attain equivalent reaction velocities are explained in terms of less efficient containment of substrate H(2)O(2) in the heme cavity arising from easier escape through the more open entrance to the lateral channel created by the smaller side chains of Gly and Ala. Inserting a Cys at position 274 resulted in the heme being covalently linked to the protein through a Cys-vinyl bond that is hypersensitive to X-ray irradiation being largely degraded within seconds of exposure to the X-ray beam. Two heme orientations, flipped along the propionate-vinyl axis, are found in the Ala, Val, and Cys variants.
-===Structure of I274C variant of E. coli KatE[] - Images 25-30===
+Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli.,Jha V, Louis S, Chelikani P, Carpena X, Donald LJ, Fita I, Loewen PC Biochemistry. 2011 Mar 29;50(12):2101-10. Epub 2011 Mar 3. PMID:21332158<ref>PMID:21332158</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3pq6" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21332158}}, adds the Publication Abstract to the page
+*[[Catalase 3D structures|Catalase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21332158 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21332158}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Escherichia coli K-12]]
-[[3pq6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PQ6 OCA].
+[[Category: Large Structures]]
+[[Category: Carpena X]]
-==Reference==
+[[Category: Chelikani P]]
-<ref group="xtra">PMID:021332158</ref><references group="xtra"/>
+[[Category: Fita I]]
-[[Category: Catalase]]
+[[Category: Jha V]]
-[[Category: Escherichia coli]]
+[[Category: Loewen PC]]
-[[Category: Carpena, X.]]
+[[Category: Louis S]]
-[[Category: Chelikani, P.]]
-[[Category: Fita, I.]]
-[[Category: Jha, V.]]
-[[Category: Loewen, P C.]]
-[[Category: Louis, S.]]
-[[Category: Catalase]]
-[[Category: Heme orientation]]
-[[Category: I274c variant]]
-[[Category: Oxidoreductase]]
-[[Category: X-ray damage]]