3ppg: Difference between revisions

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-[[Image:3ppg.png|left|200px]]
-<!--
+==Crystal structure of the Candida albicans methionine synthase by surface entropy reduction, alanine variant with zinc==
-The line below this paragraph, containing "STRUCTURE_3ppg", creates the "Structure Box" on the page.
+<StructureSection load='3ppg' size='340' side='right'caption='[[3ppg]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3ppg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PPG FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_3ppg|  PDB=3ppg  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ppg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ppg OCA], [https://pdbe.org/3ppg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ppg RCSB], [https://www.ebi.ac.uk/pdbsum/3ppg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ppg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Fungal methionine synthase, Met6p, transfers a methyl group from 5-methyl-tetrahydrofolate to homocysteine to generate methionine. The enzyme is essential to fungal growth and is a potential anti-fungal drug design target. We have characterized the enzyme from the pathogen Candida albicans but were unable to crystallize it in native form. We converted Lys103, Lys104, and Glu107 all to Tyr (Met6pY), Thr (Met6pT) and Ala (Met6pA). All variants showed wild-type kinetic activity and formed useful crystals, each with unique crystal packing. In each case the mutated residues participated in beneficial crystal contacts. We have solved the three structures at 2.0-2.8A resolution and analyzed crystal packing, active-site residues, and similarity to other known methionine synthase structures. C. albicans Met6p has a two domain structure with each of the domains having a (betaalpha)(8)-barrel fold. The barrels are arranged face-to-face and the active site is located in a cleft between the two domains. Met6p utilizes a zinc ion for catalysis that is bound in the C-terminal domain and ligated by four conserved residues: His657, Cys659, Glu679 and Cys739.
-===Crystal structure of the Candida albicans methionine synthase by surface entropy reduction, alanine variant with zinc===
+Structure of Candida albicans methionine synthase determined by employing surface residue mutagenesis.,Ubhi D, Kavanagh KL, Monzingo AF, Robertus JD Arch Biochem Biophys. 2011 Sep 1;513(1):19-26. doi:, 10.1016/j.abb.2011.06.002. Epub 2011 Jun 12. PMID:21689631<ref>PMID:21689631</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3ppg" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21689631}}, adds the Publication Abstract to the page
+*[[Methionine synthase 3D structures|Methionine synthase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21689631 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21689631}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3ppg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPG OCA].
-==Reference==
-<ref group="xtra">PMID:021689631</ref><references group="xtra"/>
-[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]
 [[Category: Candida albicans]]
-[[Category: Kavanagh, K.]]
+[[Category: Large Structures]]
-[[Category: Monzingo, A F.]]
+[[Category: Kavanagh K]]
-[[Category: Robertus, J D.]]
+[[Category: Monzingo AF]]
-[[Category: Ubhi, D.]]
+[[Category: Robertus JD]]
-[[Category: Cobalamin-independent]]
+[[Category: Ubhi D]]
-[[Category: Metalloproteinase]]
-[[Category: Methyltransferase]]
-[[Category: Surface entropy reduction]]
-[[Category: Transferase]]