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[[Image:3pl8.jpg|left|200px]]


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==Pyranose 2-oxidase H167A complex with 3-deoxy-3-fluoro-beta-D-glucose==
The line below this paragraph, containing "STRUCTURE_3pl8", creates the "Structure Box" on the page.
<StructureSection load='3pl8' size='340' side='right'caption='[[3pl8]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3pl8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trametes_ochracea Trametes ochracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PL8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=G3F:3-DEOXY-3-FLUORO-BETA-D-GLUCOPYRANOSE'>G3F</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
{{STRUCTURE_3pl8|  PDB=3pl8  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pl8 OCA], [https://pdbe.org/3pl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pl8 RCSB], [https://www.ebi.ac.uk/pdbsum/3pl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pl8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7ZA32_TRAOC Q7ZA32_TRAOC]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Trametes multicolor pyranose 2-oxidase (P2O) is a flavoprotein oxidase that oxidizes d-glucose at C2 to 2-keto-d-glucose by a highly regioselective mechanism. In this work, fluorinated sugar substrates were used as mechanistic probes to investigate the basis of regioselectivity in P2O. Although frequently used to study the mechanisms of glycoside hydrolases, our work provides the first example of applying these probes to sugar oxidoreductases. Our previous structure of the P2O mutant H167A in complex with the slow substrate 2-deoxy-2-fluoro-d-glucose showed a substrate-binding mode compatible with oxidation at C3. To accommodate the sugar, a gating segment, (454)FSY(456), in the substrate recognition loop partly unfolded to create a spacious and more polar active site that is distinct from the closed state of P2O. The crystal structure presented here shows that the preferred C2 oxidation where an ordered complex of P2O H167A with 3-deoxy-3-fluoro-d-glucose at 1.35 A resolution was successfully trapped. In this semi-open C2-oxidation complex, the substrate recognition loop tightens to form an optimized substrate complex stabilized by interactions between Asp452 and glucose O4, as well as Tyr456 and the glucose O6 group, interactions that are not possible when glucose is positioned for oxidation at C3. The different conformations of the (454)FSY(456) gating segment in the semi-open and closed states induce backbone and side-chain movements of Thr169 and Asp452 that add further differential stabilization to the individual states. We expect the semi-open state (C2-oxidation state) and closed state to be good approximations of the active-site structure during the reductive half-reaction (sugar oxidation) and oxidative half-reaction (O(2) reduction).


===Pyranose 2-oxidase H167A complex with 3-deoxy-3-fluoro-beta-D-glucose===
Regioselective control of beta-d-glucose oxidation by pyranose 2-oxidase is intimately coupled to conformational degeneracy.,Tan TC, Haltrich D, Divne C J Mol Biol. 2011 Jun 17;409(4):588-600. Epub 2011 Apr 15. PMID:21515286<ref>PMID:21515286</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3pl8" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[3pl8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trametes_ochracea Trametes ochracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PL8 OCA].
*[[Pyranose oxidase|Pyranose oxidase]]
[[Category: Pyranose oxidase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trametes ochracea]]
[[Category: Trametes ochracea]]
[[Category: Divne, C.]]
[[Category: Divne C]]
[[Category: Tan, T C.]]
[[Category: Tan TC]]

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