3ph2: Difference between revisions
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<StructureSection load='3ph2' size='340' side='right'caption='[[3ph2]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='3ph2' size='340' side='right'caption='[[3ph2]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ph2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3ph2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_laminosum Phormidium laminosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PH2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ph2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ph2 OCA], [https://pdbe.org/3ph2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ph2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ph2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ph2 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ph2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ph2 OCA], [https://pdbe.org/3ph2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ph2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ph2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ph2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/F2Z292_PHOLA F2Z292_PHOLA] Functions as an electron carrier between membrane-bound cytochrome b6-f and photosystem I in oxygenic photosynthesis.[HAMAP-Rule:MF_00594] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Cytochrome f 3D structures|Cytochrome f 3D structures]] | *[[Cytochrome f 3D structures|Cytochrome f 3D structures]] | ||
== References == | == References == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Phormidium laminosum]] | ||
[[Category: Worrall | [[Category: Worrall JAR]] | ||
Latest revision as of 12:54, 6 September 2023
Structure of the imidazole-adduct of the Phormidium laminosum cytochrome c6 Q51V variantStructure of the imidazole-adduct of the Phormidium laminosum cytochrome c6 Q51V variant
Structural highlights
FunctionF2Z292_PHOLA Functions as an electron carrier between membrane-bound cytochrome b6-f and photosystem I in oxygenic photosynthesis.[HAMAP-Rule:MF_00594] Publication Abstract from PubMedThe amino acid at position 51 in the cytochrome c (6) family is responsible for modulating over 100 mV of heme midpoint redox potential. As part of the present work, the X-ray structure of the imidazole adduct of the photosynthetic cytochrome c (6) Q51V variant from Phormidium laminosum has been determined. The structure reveals the axial Met ligand is dissociated from the heme iron but remains inside the heme pocket and the Omega-loop housing the Met ligand is stabilized through polar interactions with the imidazole and heme propionate-6. The latter is possible owing to a 180 degrees rotation of both heme propionates upon imidazole binding. From equilibrium and kinetic studies, a Val residue at position 51 increases the stability of the Fe-S(Met) interaction and also affects the dynamics associated with imidazole binding. In this respect, the k (obs) for imidazole binding to Arabidopsis thaliana cytochrome c (6A), which has a Val at the position equivalent to position 51 in photosynthetic cytochrome c (6), was found to be independent of imidazole concentration, indicating that the binding process is limited by the Met dissociation rate constant (about 1 s(-1)). For the cytochrome c (6) Q51V variant, imidazole binding was suppressed in comparison with the wild-type protein and the V52Q variant of cytochrome c (6A) was found to bind imidazole readily. We conclude that the residue type at position 51/52 in the cytochrome c (6) family is additionally responsible for tuning the stability of the heme iron-Met bond and the dynamic properties of the ferric protein fold associated with endogenous ligand binding. Structural and kinetic studies of imidazole binding to two members of the cytochrome c (6) family reveal an important role for a conserved heme pocket residue.,Rajagopal BS, Wilson MT, Bendall DS, Howe CJ, Worrall JA J Biol Inorg Chem. 2011 Jan 26. PMID:21267610[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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