3ol9: Difference between revisions

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-[[Image:3ol9.png|left|200px]]
-<!--
+==Poliovirus polymerase elongation complex with 3'-deoxy-CTP==
-The line below this paragraph, containing "STRUCTURE_3ol9", creates the "Structure Box" on the page.
+<StructureSection load='3ol9' size='340' side='right'caption='[[3ol9]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3ol9]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_poliovirus_1 Human poliovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OL9 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=O2C:3-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>O2C</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_3ol9|  PDB=3ol9  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ol9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ol9 OCA], [https://pdbe.org/3ol9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ol9 RCSB], [https://www.ebi.ac.uk/pdbsum/3ol9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ol9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/B3VQP5_9ENTO B3VQP5_9ENTO]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Positive-strand RNA viruses include a large number of human and animal pathogens whose essential RNA-dependent RNA polymerases (RdRPs) share a structurally homologous core with an encircled active site. RdRPs are targets for antiviral drug development, but these efforts are hindered by limited structural information about the RdRP catalytic cycle. To further our understanding of RdRP function, we assembled, purified, and then crystallized poliovirus elongation complexes after multiple rounds of nucleotide incorporation. Here we present structures capturing the active polymerase and its nucleotide triphosphate complexes in four distinct states, leading us to propose a six-state catalytic cycle involving residues that are highly conserved among positive-strand RNA virus RdRPs. The structures indicate that RdRPs use a fully prepositioned templating base for nucleotide recognition and close their active sites for catalysis using a novel structural rearrangement in the palm domain. The data also suggest that translocation by RDRPs may not be directly linked to the conformational changes responsible for active site closure and reopening.
-===Poliovirus polymerase elongation complex with 3'-deoxy-CTP===
+Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.,Gong P, Peersen OB Proc Natl Acad Sci U S A. 2010 Dec 10. PMID:21148772<ref>PMID:21148772</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3ol9" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21148772}}, adds the Publication Abstract to the page
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21148772 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21148772}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3ol9]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_poliovirus_1 Human poliovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OL9 OCA].
-==Reference==
-<ref group="xtra">PMID:21148772</ref><references group="xtra"/>
 [[Category: Human poliovirus 1]]
-[[Category: RNA-directed RNA polymerase]]
+[[Category: Large Structures]]
-[[Category: Gong, P.]]
+[[Category: Gong P]]
-[[Category: Peersen, O B.]]
+[[Category: Peersen OB]]