3oc9: Difference between revisions

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-{{STRUCTURE_3oc9|  PDB=3oc9  |  SCENE=  }}
-===Crystal structure of putative UDP-N-acetylglucosamine pyrophosphorylase from Entamoeba histolytica===
-==About this Structure==
+==Crystal structure of putative UDP-N-acetylglucosamine pyrophosphorylase from Entamoeba histolytica==
-[[3oc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OC9 OCA].
+<StructureSection load='3oc9' size='340' side='right'caption='[[3oc9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-[[Category: Entamoeba histolytica]]
+== Structural highlights ==
-[[Category: UDP-N-acetylglucosamine diphosphorylase]]
+<table><tr><td colspan='2'>[[3oc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica_HM-1:IMSS Entamoeba histolytica HM-1:IMSS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OC9 FirstGlance]. <br>
-[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-[[Category: Amoebic dysentery]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-[[Category: Amoebic liver abscess]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oc9 OCA], [https://pdbe.org/3oc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oc9 RCSB], [https://www.ebi.ac.uk/pdbsum/3oc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oc9 ProSAT]</span></td></tr>
-[[Category: Anaerobic parasitic protozoan]]
+</table>
-[[Category: Cyst]]
+== Function ==
-[[Category: Nucleotidyl transferase]]
+[https://www.uniprot.org/uniprot/C4M036_ENTH1 C4M036_ENTH1]
-[[Category: Seattle structural genomics center for infectious disease]]
+== Evolutionary Conservation ==
-[[Category: Ssgcid]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Structural genomic]]
+Check<jmol>
-[[Category: Transferase]]
+  <jmolCheckbox>
-[[Category: Udp-n-acetylglucosamine diphosphorylase]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/3oc9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3oc9 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Uridine diphosphate N-acetylglucosamine pyrophosphorylase (UAP) catalyzes the final step in the synthesis of UDP-GlcNAc, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. Small-molecule inhibitors have been developed against UAP from Trypanosoma brucei that target an allosteric pocket to provide selectivity over the human enzyme. A 1.8 A resolution crystal structure was determined of UAP from Entamoeba histolytica, an anaerobic parasitic protozoan that causes amoebic dysentery. Although E. histolytica UAP exhibits the same three-domain global architecture as other UAPs, it appears to lack three alpha-helices at the N-terminus and contains two amino acids in the allosteric pocket that make it appear more like the enzyme from the human host than that from the other parasite T. brucei. Thus, allosteric inhibitors of T. brucei UAP are unlikely to target Entamoeba UAPs.
+Structure of uridine diphosphate N-acetylglucosamine pyrophosphorylase from Entamoeba histolytica.,Edwards TE, Gardberg AS, Phan IQ, Zhang Y, Staker BL, Myler PJ, Lorimer DD Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):560-5. doi:, 10.1107/S2053230X1500179X. Epub 2015 Apr 21. PMID:25945709<ref>PMID:25945709</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3oc9" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Entamoeba histolytica HM-1:IMSS]]
+[[Category: Large Structures]]