3o8b: Difference between revisions

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-[[Image:3o8b.png|left|200px]]
-<!--
+==Visualizing ATP-dependent RNA Translocation by the NS3 Helicase from HCV==
-The line below this paragraph, containing "STRUCTURE_3o8b", creates the "Structure Box" on the page.
+<StructureSection load='3o8b' size='340' side='right'caption='[[3o8b]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3o8b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_C_virus_subtype_1b Hepatitis C virus subtype 1b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O8B FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_3o8b|  PDB=3o8b  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o8b OCA], [https://pdbe.org/3o8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o8b RCSB], [https://www.ebi.ac.uk/pdbsum/3o8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o8b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q99AU2_9HEPC Q99AU2_9HEPC]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The structural mechanism by which nonstructural protein 3 (NS3) from the hepatitis C virus (HCV) translocates along RNA is currently unknown. HCV NS3 is an ATP-dependent motor protein essential for viral replication and a member of the superfamily 2 helicases. Crystallographic analysis using a labeled RNA oligonucleotide allowed us to unambiguously track the positional changes of RNA bound to full-length HCV NS3 during two discrete steps of the ATP hydrolytic cycle. The crystal structures of HCV NS3, NS3 bound to bromine-labeled RNA, and a tertiary complex of NS3 bound to labeled RNA and a non-hydrolyzable ATP analog provide a direct view of how large domain movements resulting from ATP binding and hydrolysis allow the enzyme to translocate along the phosphodiester backbone. While directional translocation of HCV NS3 by a single base pair per ATP hydrolyzed is observed, the 3' end of the RNA does not shift register with respect to a conserved tryptophan residue, supporting a "spring-loading" mechanism that leads to larger steps by the enzyme as it moves along a nucleic acid substrate.
-===Visualizing ATP-dependent RNA Translocation by the NS3 Helicase from HCV===
+Visualizing ATP-Dependent RNA Translocation by the NS3 Helicase from HCV.,Appleby TC, Anderson R, Fedorova O, Pyle AM, Wang R, Liu X, Brendza KM, Somoza JR J Mol Biol. 2010 Dec 9. PMID:21145896<ref>PMID:21145896</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3o8b" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21145896}}, adds the Publication Abstract to the page
+*[[Helicase 3D structures|Helicase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21145896 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21145896}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Hepatitis C virus subtype 1b]]
-[[3o8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O8B OCA].
+[[Category: Large Structures]]
+[[Category: Appleby TC]]
-==Reference==
+[[Category: Somoza JR]]
-<ref group="xtra">PMID:21145896</ref><references group="xtra"/>
-[[Category: Hepatitis c virus]]
-[[Category: Appleby, T C.]]
-[[Category: Somoza, J R.]]