3ny6: Difference between revisions

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-[[Image:3ny6.jpg|left|200px]]
-<!--
+==Catalytic fragment of cholix toxin from vibrio cholerae in complex with inhibitor V30==
-The line below this paragraph, containing "STRUCTURE_3ny6", creates the "Structure Box" on the page.
+<StructureSection load='3ny6' size='340' side='right'caption='[[3ny6]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3ny6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NY6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=V30:2-[(5,6-DIMETHYL-4-OXO-3,4-DIHYDROTHIENO[2,3-D]PYRIMIDIN-2-YL)SULFANYL]-N-(2-HYDROXYETHYL)ACETAMIDE'>V30</scene></td></tr>
-{{STRUCTURE_3ny6|  PDB=3ny6  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ny6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ny6 OCA], [https://pdbe.org/3ny6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ny6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ny6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ny6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CHXA_VIBCL CHXA_VIBCL] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The mono-ADP-ribosyltransferase toxins are bacterial virulence factors that contribute to many disease states in plants, animals and humans. These toxins function as enzymes that target various host proteins and covalently attach an ADP-ribose moiety that alters target protein function. We tested compounds from a virtual screen of commercially available compounds combined with a directed PARP inhibitor library and found several compounds that bind tightly and inhibit toxins from Pseudomonas aeruginosa and Vibrio cholerae. The most efficacious compounds completely protected human lung epithelial cells against the cytotoxicity of these bacterial virulence factors. Moreover, we determined high-resolution crystal structures of the best inhibitors in complex with cholix toxin to reveal important criteria for inhibitor binding and mechanism of action. These results provide new insight in antivirulence compound development for treating many bacterial diseases.
-===Catalytic fragment of cholix toxin from vibrio cholerae in complex with inhibitor V30===
+Newly Discovered and Characterized Antivirulence Compounds inhibit Bacterial Mono-ADP-ribosyltransferase Toxins.,Turgeon Z, Jorgensen R, Visschedyk D, Edwards PR, Legree S, McGregor C, Fieldhouse RJ, Mangroo D, Schapira M, Merrill AR Antimicrob Agents Chemother. 2010 Dec 6. PMID:21135177<ref>PMID:21135177</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3ny6" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21135177}}, adds the Publication Abstract to the page
+*[[Exotoxin 3D structures|Exotoxin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21135177 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21135177}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[3ny6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NY6 OCA].
-==Reference==
-<ref group="xtra">PMID:21135177</ref><references group="xtra"/>
 [[Category: Vibrio cholerae]]
-[[Category: Jorgensen, R.]]
+[[Category: Jorgensen R]]
-[[Category: Merrill, A R.]]
+[[Category: Merrill AR]]
-[[Category: Adp-ribosyl transferase]]
-[[Category: Alpha-beta complex]]
-[[Category: Nad+ binding]]
-[[Category: Transferase]]
-[[Category: Transferase-transferase inhibitor complex]]