3nmh: Difference between revisions

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-{{Seed}}
-[[Image:3nmh.jpg|left|200px]]
-<!--
+==Crystal structure of the abscisic receptor PYL2 in complex with pyrabactin==
-The line below this paragraph, containing "STRUCTURE_3nmh", creates the "Structure Box" on the page.
+<StructureSection load='3nmh' size='340' side='right'caption='[[3nmh]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3nmh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NMH FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PYV:4-BROMO-N-(PYRIDIN-2-YLMETHYL)NAPHTHALENE-1-SULFONAMIDE'>PYV</scene></td></tr>
-{{STRUCTURE_3nmh|  PDB=3nmh  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nmh OCA], [https://pdbe.org/3nmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nmh RCSB], [https://www.ebi.ac.uk/pdbsum/3nmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nmh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PYL2_ARATH PYL2_ARATH] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.<ref>PMID:19898420</ref> <ref>PMID:19893533</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/3nmh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nmh ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The phytohormone abscisic acid (ABA) functions through a family of fourteen PYR/PYL receptors, which were identified by resistance to pyrabactin, a synthetic inhibitor of seed germination. ABA activates these receptors to inhibit type 2C protein phosphatases, such as ABI1, yet it remains unclear whether these receptors can be antagonized. Here we demonstrate that pyrabactin is an agonist of PYR1 and PYL1 but is unexpectedly an antagonist of PYL2. Crystal structures of the PYL2-pyrabactin and PYL1-pyrabactin-ABI1 complexes reveal the mechanism responsible for receptor-selective activation and inhibition, which enables us to design mutations that convert PYL1 to a pyrabactin-inhibited receptor and PYL2 to a pyrabactin-activated receptor and to identify new pyrabactin-based ABA receptor agonists. Together, our results establish a new concept of ABA receptor antagonism, illustrate its underlying mechanisms and provide a rational framework for discovering novel ABA receptor ligands.
-===Crystal structure of the abscisic receptor PYL2 in complex with pyrabactin===
+Identification and mechanism of ABA receptor antagonism.,Melcher K, Xu Y, Ng LM, Zhou XE, Soon FF, Chinnusamy V, Suino-Powell KM, Kovach A, Tham FS, Cutler SR, Li J, Yong EL, Zhu JK, Xu HE Nat Struct Mol Biol. 2010 Sep;17(9):1102-8. Epub 2010 Aug 22. PMID:20729862<ref>PMID:20729862</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3nmh" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-NMH is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMH OCA].
+*[[Abscisic acid receptor 3D structures|Abscisic acid receptor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Arabidopsis thaliana]]
-[[Category: Kovach, A.]]
+[[Category: Large Structures]]
-[[Category: Li, J.]]
+[[Category: Kovach A]]
-[[Category: Melcher, K.]]
+[[Category: Li J]]
-[[Category: Ng, L M.]]
+[[Category: Melcher K]]
-[[Category: Soon, F F.]]
+[[Category: Ng L-M]]
-[[Category: Suino-Powell, K M.]]
+[[Category: Soon F-F]]
-[[Category: Xu, H E.]]
+[[Category: Suino-Powell KM]]
-[[Category: Xu, Y.]]
+[[Category: Xu HE]]
-[[Category: Yong, E L.]]
+[[Category: Xu Y]]
-[[Category: Zhou, X E.]]
+[[Category: Yong E-L]]
-[[Category: Abscisic acid]]
+[[Category: Zhou XE]]
-[[Category: Helix-grip fold]]
-[[Category: Hormone binding]]
-[[Category: Hormone binding protein]]
-[[Category: Plant hormone receptor]]
-[[Category: Pyl2]]
-[[Category: Pyrabactin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 09:00:14 2010''