3nk4: Difference between revisions

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-[[Image:3nk4.png|left|200px]]
-<!--
+==Crystal structure of full-length sperm receptor ZP3 at 2.0 A resolution==
-The line below this paragraph, containing "STRUCTURE_3nk4", creates the "Structure Box" on the page.
+<StructureSection load='3nk4' size='340' side='right'caption='[[3nk4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3nk4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NK4 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-{{STRUCTURE_3nk4|  PDB=3nk4  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nk4 OCA], [https://pdbe.org/3nk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nk4 RCSB], [https://www.ebi.ac.uk/pdbsum/3nk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nk4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ZP3_CHICK ZP3_CHICK] Component of the zona pellucida, which mediates species-specific sperm binding. Directly binds to sperm. Important for egg fertilization.<ref>PMID:15115720</ref> <ref>PMID:20970175</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+ZP3, a major component of the zona pellucida (ZP) matrix coating mammalian eggs, is essential for fertilization by acting as sperm receptor. By retaining a propeptide that contains a polymerization-blocking external hydrophobic patch (EHP), we determined the crystal structure of an avian homolog of ZP3 at 2.0 A resolution. The structure unveils the fold of a complete ZP domain module in a homodimeric arrangement required for secretion and reveals how EHP prevents premature incorporation of ZP3 into the ZP. This suggests mechanisms underlying polymerization and how local structural differences, reflected by alternative disulfide patterns, control the specificity of ZP subunit interaction. Close relative positioning of a conserved O-glycan important for sperm binding and the hypervariable, positively selected C-terminal region of ZP3 suggests a concerted role in the regulation of species-restricted gamete recognition. Alternative conformations of the area around the O-glycan indicate how sperm binding could trigger downstream events via intramolecular signaling.
-===Crystal structure of full-length sperm receptor ZP3 at 2.0 A resolution===
+Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3.,Han L, Monne M, Okumura H, Schwend T, Cherry AL, Flot D, Matsuda T, Jovine L Cell. 2010 Oct 29;143(3):404-15. Epub 2010 Oct 21. PMID:20970175<ref>PMID:20970175</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_20970175}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3nk4" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 20970175 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20970175}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3nk4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NK4 OCA].
-==Reference==
-<ref group="xtra">PMID:20970175</ref><ref group="xtra">PMID:7418009</ref><ref group="xtra">PMID:1313375</ref><ref group="xtra">PMID:9780332</ref><ref group="xtra">PMID:10103002</ref><ref group="xtra">PMID:12021773</ref><ref group="xtra">PMID:15079052</ref><ref group="xtra">PMID:15952882</ref><ref group="xtra">PMID:19052627</ref><references group="xtra"/>
 [[Category: Gallus gallus]]
-[[Category: Jovine, L.]]
+[[Category: Large Structures]]
-[[Category: Monne , M.]]
+[[Category: Jovine L]]
-[[Category: Biodiversity]]
+[[Category: Monne M]]
-[[Category: Cell adhesion]]
-[[Category: Core-1]]
-[[Category: Egg coat]]
-[[Category: Egg-sperm interaction]]
-[[Category: Ehp]]
-[[Category: External hydrophobic patch]]
-[[Category: Extracellular matrix]]
-[[Category: Fertilization]]
-[[Category: Glycoprotein]]
-[[Category: Ihp]]
-[[Category: Immunoglobulin-like fold]]
-[[Category: Infertility]]
-[[Category: Internal hydrophobic patch]]
-[[Category: O-linked carbohydrate]]
-[[Category: Oocyte]]
-[[Category: Receptor]]
-[[Category: Secreted]]
-[[Category: Speciation]]
-[[Category: Species-specific gamete recognition]]
-[[Category: Sperm-combining site]]
-[[Category: T-antigen]]
-[[Category: Transmembrane]]
-[[Category: Vitelline envelope]]
-[[Category: Zona pellucida]]
-[[Category: Zp domain]]
-[[Category: Zp module]]