3nj1: Difference between revisions

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-[[Image:3nj1.png|left|200px]]
-<!--
+==X-ray crystal structure of the PYL2(V114I)-pyrabactin A complex==
-The line below this paragraph, containing "STRUCTURE_3nj1", creates the "Structure Box" on the page.
+<StructureSection load='3nj1' size='340' side='right'caption='[[3nj1]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3nj1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NJ1 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.948&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P2M:N-(PYRIDIN-2-YLMETHYL)NAPHTHALENE-1-SULFONAMIDE'>P2M</scene>, <scene name='pdbligand=PYV:4-BROMO-N-(PYRIDIN-2-YLMETHYL)NAPHTHALENE-1-SULFONAMIDE'>PYV</scene></td></tr>
-{{STRUCTURE_3nj1|  PDB=3nj1  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nj1 OCA], [https://pdbe.org/3nj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nj1 RCSB], [https://www.ebi.ac.uk/pdbsum/3nj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nj1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PYL2_ARATH PYL2_ARATH] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.<ref>PMID:19898420</ref> <ref>PMID:19893533</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nj/3nj1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nj1 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discover its primary target PYR1, the founding member of the PYR/PYL family of soluble ABA receptors. To understand pyrabactin's selectivity, we have taken a combined structural, chemical and genetic approach. We show that subtle differences between receptor binding pockets control ligand orientation between productive and nonproductive modes. Nonproductive binding occurs without gate closure and prevents receptor activation. Observations in solution show that these orientations are in rapid equilibrium that can be shifted by mutations to control maximal agonist activity. Our results provide a robust framework for the design of new agonists and reveal a new mechanism for agonist selectivity.
-===X-ray crystal structure of the PYL2(V114I)-pyrabactin A complex===
+Structural basis for selective activation of ABA receptors.,Peterson FC, Burgie ES, Park SY, Jensen DR, Weiner JJ, Bingman CA, Chang CE, Cutler SR, Phillips GN Jr, Volkman BF Nat Struct Mol Biol. 2010 Sep;17(9):1109-13. Epub 2010 Aug 22. PMID:20729860<ref>PMID:20729860</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3nj1" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20729860}}, adds the Publication Abstract to the page
+*[[Abscisic acid receptor 3D structures|Abscisic acid receptor 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20729860 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20729860}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3nj1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ1 OCA].
-==Reference==
-<ref group="xtra">PMID:20729860</ref><references group="xtra"/>
 [[Category: Arabidopsis thaliana]]
-[[Category: Bingman, C A.]]
+[[Category: Large Structures]]
-[[Category: Burgie, E S.]]
+[[Category: Bingman CA]]
-[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
+[[Category: Burgie ES]]
-[[Category: Cutler, S R.]]
+[[Category: Cutler SR]]
-[[Category: Jensen, D R.]]
+[[Category: Jensen DR]]
-[[Category: Peterson, F C.]]
+[[Category: Peterson FC]]
-[[Category: Phillips, G N.]]
+[[Category: Phillips Jr GN]]
-[[Category: Volkman, B F.]]
+[[Category: Volkman BF]]