3nh4: Difference between revisions

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-{{Seed}}
-[[Image:3nh4.jpg|left|200px]]
-<!--
+==Crystal structure of murine aminoacylase 3==
-The line below this paragraph, containing "STRUCTURE_3nh4", creates the "Structure Box" on the page.
+<StructureSection load='3nh4' size='340' side='right'caption='[[3nh4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3nh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NH4 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_3nh4|  PDB=3nh4  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nh4 OCA], [https://pdbe.org/3nh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nh4 RCSB], [https://www.ebi.ac.uk/pdbsum/3nh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nh4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ACY3_MOUSE ACY3_MOUSE] Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids.<ref>PMID:14656720</ref> <ref>PMID:17012540</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Trichloroethylene (TCE) is one of the most widespread environmental contaminants, which is metabolized to N-acetyl-S-1,2-dichlorovinyl-l-cysteine (NA-DCVC) before being excreted in the urine. Alternatively, NA-DCVC can be deacetylated by aminoacylase 3 (AA3), an enzyme that is highly expressed in the kidney, liver, and brain. NA-DCVC deacetylation initiates the transformation into toxic products that ultimately causes acute renal failure. AA3 inhibition is therefore a target of interest to prevent TCE induced nephrotoxicity. Here we report the crystal structure of recombinant mouse AA3 (mAA3) in the presence of its acetate byproduct and two substrates: N(alpha)-acetyl-l-tyrosine and NA-DCVC. These structures, in conjunction with biochemical data, indicated that AA3 mediates substrate specificity through van der Waals interactions providing a dynamic interaction interface, which facilitates a diverse range of substrates.
-===Crystal structure of murine aminoacylase 3===
+Structures of aminoacylase 3 in complex with acetylated substrates.,Hsieh JM, Tsirulnikov K, Sawaya MR, Magilnick N, Abuladze N, Kurtz I, Abramson J, Pushkin A Proc Natl Acad Sci U S A. 2010 Oct 4. PMID:20921362<ref>PMID:20921362</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3nh4" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20921362}}, adds the Publication Abstract to the page
+*[[Aminoacylase 3D structures|Aminoacylase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20921362 is the PubMed ID number.
+*[[Aspartoacylase 3D structures|Aspartoacylase 3D structures]]
--->
+== References ==
-{{ABSTRACT_PUBMED_20921362}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-NH4 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NH4 OCA].
+[[Category: Large Structures]]
-==Reference==
-<ref group="xtra">PMID:20921362</ref><references group="xtra"/>
 [[Category: Mus musculus]]
-[[Category: Abramson, J.]]
+[[Category: Abramson J]]
-[[Category: Abuladze, N.]]
+[[Category: Abuladze N]]
-[[Category: Hsieh, J M.]]
+[[Category: Hsieh JM]]
-[[Category: Kurtz, I.]]
+[[Category: Kurtz I]]
-[[Category: Magilnick, N.]]
+[[Category: Magilnick N]]
-[[Category: Pushkin, A.]]
+[[Category: Pushkin A]]
-[[Category: Sawaya, M R.]]
+[[Category: Sawaya MR]]
-[[Category: Tsirulnikov, K.]]
+[[Category: Tsirulnikov K]]
-[[Category: Hydrolase]]
-[[Category: Mercapturate]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:38:05 2010''