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[[Image:3ndv.png|left|200px]]


{{STRUCTURE_3ndv|  PDB=3ndv  |  SCENE=  }}
==Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with ampicillin==
 
<StructureSection load='3ndv' size='340' side='right'caption='[[3ndv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
===Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with ampicillin===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3ndv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NDV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NDV FirstGlance]. <br>
 
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
==About this Structure==
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIC:(2S,5R,6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3,3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC+ACID'>AIC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NDV:(2S,4S)-2-[(1R)-1-{[(2R)-2-AMINO-2-PHENYLACETYL]AMINO}-2-OXOETHYL]-5,5-DIMETHYL-1,3-THIAZOLIDINE-4-CARBOXYLIC+ACID'>NDV</scene></td></tr>
[[3ndv]] is a 4 chain structure of [[Aminopeptidase]] with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NDV OCA].  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ndv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ndv OCA], [https://pdbe.org/3ndv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ndv RCSB], [https://www.ebi.ac.uk/pdbsum/3ndv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ndv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>


==See Also==
==See Also==
*[[Aminopeptidase|Aminopeptidase]]
*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sphingosinicella xenopeptidilytica]]
[[Category: Sphingosinicella xenopeptidilytica]]
[[Category: Geueke, B.]]
[[Category: Geueke B]]
[[Category: Gruetter, M G.]]
[[Category: Gruetter MG]]
[[Category: Heck, T.]]
[[Category: Heck T]]
[[Category: Kohler, H P.E.]]
[[Category: Kohler H-PE]]
[[Category: Merz, T.]]
[[Category: Merz T]]
[[Category: Alpha-beta-beta-alpha sandwich]]
[[Category: Beta-aminopeptidase]]
[[Category: Beta-peptide]]
[[Category: Hydrolase-antibiotic complex]]
[[Category: N-terminal beta-aminopeptidase]]
[[Category: Ntn-hydrolase]]

Latest revision as of 12:13, 6 September 2023

Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with ampicillinCrystal structure of the N-terminal beta-aminopeptidase BapA in complex with ampicillin

Structural highlights

3ndv is a 4 chain structure with sequence from Sphingosinicella xenopeptidilytica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BAPA_SPHXN Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.[1] [2]

See Also

References

  1. ↑ Geueke B, Namoto K, Seebach D, Kohler HP. A novel beta-peptidyl aminopeptidase (BapA) from strain 3-2W4 cleaves peptide bonds of synthetic beta-tri- and beta-dipeptides. J Bacteriol. 2005 Sep;187(17):5910-7. PMID:16109932 doi:http://dx.doi.org/187/17/5910
  2. ↑ Geueke B, Heck T, Limbach M, Nesatyy V, Seebach D, Kohler HP. Bacterial beta-peptidyl aminopeptidases with unique substrate specificities for beta-oligopeptides and mixed beta,alpha-oligopeptides. FEBS J. 2006 Dec;273(23):5261-72. Epub 2006 Oct 25. PMID:17064315 doi:http://dx.doi.org/10.1111/j.1742-4658.2006.05519.x

3ndv, resolution 1.70Å

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