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==Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with pefabloc SC (AEBSF)== | ==Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with pefabloc SC (AEBSF)== | ||
<StructureSection load='3n33' size='340' side='right' caption='[[3n33]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3n33' size='340' side='right'caption='[[3n33]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3n33]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3n33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N33 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n33 OCA], [https://pdbe.org/3n33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n33 RCSB], [https://www.ebi.ac.uk/pdbsum/3n33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n33 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The beta-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleophile (Ntn) hydrolases of the DmpA-like family and has the unprecedented property of cleaving N-terminal beta-amino acid residues from peptides. We determined the crystal structures of the native (alphabeta)(4) heterooctamer and of the 153 kDa precursor homotetramer at a resolution of 1.45 and 1.8 A, respectively. These structures together with mutational analyses strongly support mechanisms for autoproteolysis and catalysis that involve residues Ser250, Ser288, and Glu290. The autoproteolytic mechanism is different from the one so far described for Ntn hydrolases. The structures together with functional data also provide insight into the discriminating features of the active site cleft that determine substrate specificity. | |||
Autoproteolytic and catalytic mechanisms for the beta-aminopeptidase BapA--a member of the Ntn hydrolase family.,Merz T, Heck T, Geueke B, Mittl PR, Briand C, Seebach D, Kohler HP, Grutter MG Structure. 2012 Nov 7;20(11):1850-60. doi: 10.1016/j.str.2012.07.017. Epub 2012, Sep 12. PMID:22980995<ref>PMID:22980995</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3n33" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Aminopeptidase|Aminopeptidase]] | *[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Sphingosinicella xenopeptidilytica]] | [[Category: Sphingosinicella xenopeptidilytica]] | ||
[[Category: Geueke | [[Category: Geueke B]] | ||
[[Category: Gruetter | [[Category: Gruetter MG]] | ||
[[Category: Heck | [[Category: Heck T]] | ||
[[Category: Kohler | [[Category: Kohler H-P]] | ||
[[Category: Merz | [[Category: Merz T]] | ||
Latest revision as of 12:07, 6 September 2023
Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with pefabloc SC (AEBSF)Crystal structure of the N-terminal beta-aminopeptidase BapA in complex with pefabloc SC (AEBSF)
Structural highlights
FunctionBAPA_SPHXN Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.[1] [2] Publication Abstract from PubMedThe beta-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleophile (Ntn) hydrolases of the DmpA-like family and has the unprecedented property of cleaving N-terminal beta-amino acid residues from peptides. We determined the crystal structures of the native (alphabeta)(4) heterooctamer and of the 153 kDa precursor homotetramer at a resolution of 1.45 and 1.8 A, respectively. These structures together with mutational analyses strongly support mechanisms for autoproteolysis and catalysis that involve residues Ser250, Ser288, and Glu290. The autoproteolytic mechanism is different from the one so far described for Ntn hydrolases. The structures together with functional data also provide insight into the discriminating features of the active site cleft that determine substrate specificity. Autoproteolytic and catalytic mechanisms for the beta-aminopeptidase BapA--a member of the Ntn hydrolase family.,Merz T, Heck T, Geueke B, Mittl PR, Briand C, Seebach D, Kohler HP, Grutter MG Structure. 2012 Nov 7;20(11):1850-60. doi: 10.1016/j.str.2012.07.017. Epub 2012, Sep 12. PMID:22980995[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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