3n1c: Difference between revisions

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-[[Image:3n1c.png|left|200px]]
-<!--
+==Crystal structure of the phosphofructokinase-2 from Escherichia coli in complex with fructose-6-phosphate==
-The line below this paragraph, containing "STRUCTURE_3n1c", creates the "Structure Box" on the page.
+<StructureSection load='3n1c' size='340' side='right'caption='[[3n1c]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3n1c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N1C FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene></td></tr>
-{{STRUCTURE_3n1c|  PDB=3n1c  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n1c OCA], [https://pdbe.org/3n1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n1c RCSB], [https://www.ebi.ac.uk/pdbsum/3n1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n1c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PFKB_ECOLI PFKB_ECOLI] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.<ref>PMID:16866375</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Substrate inhibition by ATP is a regulatory feature of the phosphofructokinases isoenzymes from E. coli (Pfk-1 and Pfk-2). Under gluconeogenic conditions, the loss of this regulation in Pfk-2 causes substrate cycling of fructose-6-P and futile consumption of ATP delaying growth. In the present work we have broached the mechanism of ATP-induced inhibition of Pfk-2 from both structural and kinetic perspectives. The crystal structure of Pfk-2 in complex with fructose-6-P is reported to a resolution of 2 A. The comparison of this structure with the previously reported inhibited form of the enzyme suggests a negative interplay between fructose-6-P binding and allosteric binding of MgATP. Initial velocity experiments show a linear increase of the apparent K0.5 for fructose-6-P and a decrease in the apparent kcat as a function of MgATP concentration. These effects occur simultaneously with the induction of a sigmoidal kinetic behavior (nH approx. 2). Differences and resemblances in the patterns of fructose-6-P binding and the mechanism of inhibition are discussed for Pfk-1 and Pfk-2, as an example of evolutionary convergence, since these enzymes do not share a common ancestor.
-===Crystal structure of the phosphofructokinase-2 from Escherichia coli in complex with fructose-6-phosphate===
+The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-P: kinetic and structural analysis of the allosteric ATP inhibition.,Cabrera R, Baez M, Pereira HM, Caniuguir A, Garratt RC, Babul J J Biol Chem. 2010 Dec 8. PMID:21147773<ref>PMID:21147773</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3n1c" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21147773}}, adds the Publication Abstract to the page
+*[[Phosphofructokinase 3D structures|Phosphofructokinase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21147773 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21147773}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Escherichia coli K-12]]
-[[3n1c]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N1C OCA].
+[[Category: Large Structures]]
+[[Category: Babul J]]
-==Reference==
+[[Category: Cabrera R]]
-<ref group="xtra">PMID:21147773</ref><references group="xtra"/>
+[[Category: Caniuguir A]]
-[[Category: 6-phosphofructokinase]]
+[[Category: Garratt RC]]
-[[Category: Escherichia coli]]
+[[Category: Pereira HM]]
-[[Category: Babul, J.]]
-[[Category: Cabrera, R.]]
-[[Category: Caniuguir, A.]]
-[[Category: Garratt, R C.]]
-[[Category: Pereira, H M.]]