3mp9: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{STRUCTURE_3mp9|  PDB=3mp9  |  SCENE=  }}
-===Structure of Streptococcal protein G B1 domain at pH 3.0===
-{{ABSTRACT_PUBMED_20715051}}
-==Function==
+==Structure of Streptococcal protein G B1 domain at pH 3.0==
-[[http://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG]] Binds to the constant Fc region of IgG with high affinity.
+<StructureSection load='3mp9' size='340' side='right'caption='[[3mp9]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3mp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MP9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mp9 OCA], [https://pdbe.org/3mp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mp9 RCSB], [https://www.ebi.ac.uk/pdbsum/3mp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mp9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We report chemical shifts for H(N), N, and C' nuclei in the His-tagged B1 domain of protein G (GB1) over a range of pH values from pH 2.0 to 9.0, which fit well to standard pH-dependent equations. We also report a 1.2 A resolution crystal structure of GB1 at pH 3.0. Comparison of this crystal structure with published crystal structures at higher pHs provides details of the structural changes in GB1 associated with protonation of the carboxylate groups, in particular a conformational change in the C-terminus of the protein at low pH. An additional change described recently is not seen in the crystal structure because of crystal contacts. We show that the pH-dependent changes in chemical shifts can be almost entirely understood based on structural changes, thereby providing insight into the relationship between structure and chemical shift. In particular, we describe through-bond effects extending up to five bonds, affecting N and C' but not H(N); through-space effects of carboxylates, which fit well to a simple electric field model; and effects due to conformational change, which have a similar magnitude to many of the direct effects. Finally, we discuss cooperative effects, demonstrating a lack of cooperative unfolding in the helix, and the existence of a beta-sheet "iceberg" extending over three of the four strands. This study therefore extends the application of chemical shifts to understanding protein structure.
-==About this Structure==
+Structural origins of pH-dependent chemical shifts in the B1 domain of protein G.,Tomlinson JH, Green VL, Baker PJ, Williamson MP Proteins. 2010 Nov 1;78(14):3000-16. PMID:20715051<ref>PMID:20715051</ref>
-[[3mp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._'group_g' Streptococcus sp. 'group g']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP9 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:020715051</ref><references group="xtra"/><references/>
+</div>
-[[Category: Streptococcus sp. 'group g']]
+<div class="pdbe-citations 3mp9" style="background-color:#fffaf0;"></div>
-[[Category: Baker, P J.]]
-[[Category: Green, V L.]]
+==See Also==
-[[Category: Tomlinson, J H.]]
+*[[Protein G|Protein G]]
-[[Category: Williamson, M P.]]
+== References ==
-[[Category: Igg-binding protein]]
+<references/>
-[[Category: Protein binding]]
+__TOC__
-[[Category: Protein g]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptococcus sp. 'group G']]
+[[Category: Baker PJ]]
+[[Category: Green VL]]
+[[Category: Tomlinson JH]]
+[[Category: Williamson MP]]