3lg0: Difference between revisions
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==Structure of Plasmodium falciparum ornithine delta-aminotransferase== | ==Structure of Plasmodium falciparum ornithine delta-aminotransferase== | ||
<StructureSection load='3lg0' size='340' side='right' caption='[[3lg0]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='3lg0' size='340' side='right'caption='[[3lg0]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3lg0]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3lg0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_CDC/Honduras Plasmodium falciparum CDC/Honduras]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LG0 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lg0 OCA], [https://pdbe.org/3lg0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lg0 RCSB], [https://www.ebi.ac.uk/pdbsum/3lg0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lg0 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/OAT_PLAF7 OAT_PLAF7] The enzyme has a very narrow substrate specificity and can only catalyze the transamination of alpha-ketoglutarate with ornithine or N-acetylornithine and of glutamate-5-semialdehyde with glutamate and alanine. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/3lg0_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/3lg0_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 33: | Line 32: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Plasmodium falciparum CDC/Honduras]] | ||
[[Category: Becker | [[Category: Becker K]] | ||
[[Category: Fritz-Wolf | [[Category: Fritz-Wolf K]] | ||
[[Category: Jortzik | [[Category: Jortzik E]] | ||
[[Category: Stumpf | [[Category: Stumpf M]] | ||
Latest revision as of 11:36, 6 September 2023
Structure of Plasmodium falciparum ornithine delta-aminotransferaseStructure of Plasmodium falciparum ornithine delta-aminotransferase
Structural highlights
FunctionOAT_PLAF7 The enzyme has a very narrow substrate specificity and can only catalyze the transamination of alpha-ketoglutarate with ornithine or N-acetylornithine and of glutamate-5-semialdehyde with glutamate and alanine. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedOrnithine delta-aminotransferase (OAT) of the malaria parasite Plasmodium falciparum catalyzes the reversible conversion of ornithine into glutamate-5-semialdehyde and glutamate and is-in contrast to its human counterpart-activated by thioredoxin (Trx) by a factor of 10. Trx, glutaredoxin, and plasmoredoxin are redox-active proteins that play a crucial role in the maintenance and control of redox reactions, and were shown to interact with P. falciparum OAT. OAT, which is involved in ornithine homeostasis and proline biosynthesis, is essential for mitotic cell division in rapidly growing cells, thus representing a potential target for chemotherapeutic intervention. Here we report the three-dimensional crystal structure of P. falciparum OAT at 2.3 A resolution. The overall structure is very similar to that of the human OAT. However, in plasmodial OAT, the loop involved in substrate binding contains two cysteine residues, which are lacking in human OAT. Site-directed mutagenesis of these cysteines and functional analysis demonstrated that Cys154 and Cys163 mediate the interaction with Trx. Interestingly, the Cys154-->Ser mutant has a strongly reduced specific activity, most likely due to impaired binding of ornithine. Cys154 and Cys163 are highly conserved in Plasmodium but do not exist in other organisms, suggesting that redox regulation of OAT by Trx is specific for malaria parasites. Plasmodium might require a tight Trx-mediated control of OAT activity for coordinating ornithine homeostasis, polyamine synthesis, proline synthesis, and mitotic cell division. Redox regulation of Plasmodium falciparum ornithine delta-aminotransferase.,Jortzik E, Fritz-Wolf K, Sturm N, Hipp M, Rahlfs S, Becker K J Mol Biol. 2010 Sep 17;402(2):445-59. Epub 2010 Jul 29. PMID:20673832[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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