3fm7: Difference between revisions

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-[[Image:3fm7.png|left|200px]]
-<!--
+==Quaternary Structure of Drosophila melanogaster IC/Tctex-1/LC8; Allosteric Interactions of Dynein Light Chains with Dynein Intermediate Chain==
-The line below this paragraph, containing "STRUCTURE_3fm7", creates the "Structure Box" on the page.
+<StructureSection load='3fm7' size='340' side='right'caption='[[3fm7]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3fm7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FM7 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fm7 OCA], [https://pdbe.org/3fm7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fm7 RCSB], [https://www.ebi.ac.uk/pdbsum/3fm7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fm7 ProSAT]</span></td></tr>
-{{STRUCTURE_3fm7|  PDB=3fm7  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DYLT_DROME DYLT_DROME] Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Required for spermatid differentiation. Is not required for polarized transport in rhabdomere development and appears to be a non-essential component of the cytoplasmic dynein complex.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/3fm7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fm7 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Dynein light chains are thought to increase binding efficiency of dynein intermediate chain to both dynein heavy chain and dynactin, but their exact role is not clear. Isothermal titration calorimetry and x-ray crystallography reported herein indicate that multivalency effects underlie efficient dynein assembly and regulation. For a ternary complex of a 60-amino acid segment of dynein intermediate chain (IC) bound to two homodimeric dynein light chains Tctex1 and LC8, there is a 50-fold affinity enhancement for the second light chain binding. For a designed IC construct containing two LC8 sites, observed the 1000-fold enhancement reflects a remarkably pure entropic chelate effect of a magnitude commensurate with theoretical predictions. The lower enhancement in wild-type IC is attributed to unfavorable free energy changes associated with incremental interactions of IC with Tctex1. Our results show assembled dynein IC as an elongated, flexible polybivalent duplex, and suggest that polybivalency is an important general mechanism for constructing stable yet reversible and functionally versatile complexes.
-===Quaternary Structure of Drosophila melanogaster IC/Tctex-1/LC8; Allosteric Interactions of Dynein Light Chains with Dynein Intermediate Chain===
+Multivalency in the assembly of intrinsically disordered Dynein intermediate chain.,Hall J, Karplus PA, Barbar E J Biol Chem. 2009 Nov 27;284(48):33115-21. Epub 2009 Sep 16. PMID:19759397<ref>PMID:19759397</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_19759397}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3fm7" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 19759397 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_19759397}}
-==About this Structure==
-[[3fm7]] is a 6 chain structure of [[Dynein]] with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FM7 OCA].
 ==See Also==
 *[[Dynein|Dynein]]
-*[[Dynein light and intermediate chain|Dynein light and intermediate chain]]
+*[[Dynein 3D structures|Dynein 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019759397</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Barbar, E J.]]
+[[Category: Large Structures]]
-[[Category: Hall, J D.]]
+[[Category: Barbar EJ]]
-[[Category: Karplus, P A.]]
+[[Category: Hall JD]]
-[[Category: Contractile protein]]
+[[Category: Karplus PA]]
-[[Category: Cytoplasmic dynein]]
-[[Category: Dynein]]
-[[Category: Dynein cargo attachment complex]]
-[[Category: Dynein light chain]]
-[[Category: Ic]]
-[[Category: Intermediate chain]]
-[[Category: Lc8]]
-[[Category: Light chain 8]]
-[[Category: Light chain tctex-1]]
-[[Category: Lysosome]]
-[[Category: Membrane]]
-[[Category: Microtubule]]
-[[Category: Motor protein]]
-[[Category: Nucleus]]
-[[Category: Quaternary structure]]
-[[Category: Tctex]]
-[[Category: Wd repeat]]