3f3c: Difference between revisions

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-{{Seed}}
-[[Image:3f3c.png|left|200px]]
-<!--
+==Crystal structure of LeuT bound to 4-Fluoro-L-Phenylalanine and sodium==
-The line below this paragraph, containing "STRUCTURE_3f3c", creates the "Structure Box" on the page.
+<StructureSection load='3f3c' size='340' side='right'caption='[[3f3c]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3f3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F3C FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PFF:4-FLUORO-L-PHENYLALANINE'>PFF</scene></td></tr>
-{{STRUCTURE_3f3c|  PDB=3f3c  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f3c OCA], [https://pdbe.org/3f3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f3c RCSB], [https://www.ebi.ac.uk/pdbsum/3f3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f3c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/3f3c_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f3c ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Secondary transporters are workhorses of cellular membranes, catalyzing the movement of small molecules and ions across the bilayer and coupling substrate passage to ion gradients. However, the conformational changes that accompany substrate transport, the mechanism by which a substrate moves through the transporter, and principles of competitive inhibition remain unclear. We used crystallographic and functional studies on the leucine transporter (LeuT), a model for neurotransmitter sodium symporters, to show that various amino acid substrates induce the same occluded conformational state and that a competitive inhibitor, tryptophan (Trp), traps LeuT in an open-to-out conformation. In the Trp complex, the extracellular gate residues arginine 30 and aspartic acid 404 define a second weak binding site for substrates or inhibitors as they permeate from the extracellular solution to the primary substrate site, which demonstrates how residues that participate in gating also mediate permeation.
-===Crystal structure of LeuT bound to 4-Fluoro-L-Phenylalanine and sodium===
+A competitive inhibitor traps LeuT in an open-to-out conformation.,Singh SK, Piscitelli CL, Yamashita A, Gouaux E Science. 2008 Dec 12;322(5908):1655-61. PMID:19074341<ref>PMID:19074341</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3f3c" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_19074341}}, adds the Publication Abstract to the page
+*[[Leucine transporter|Leucine transporter]]
-(as it appears on PubMed at http://www.pubmed.gov), where 19074341 is the PubMed ID number.
+*[[Symporter 3D structures|Symporter 3D structures]]
--->
+== References ==
-{{ABSTRACT_PUBMED_19074341}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-F3C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F3C OCA].
-==Reference==
-A competitive inhibitor traps LeuT in an open-to-out conformation., Singh SK, Piscitelli CL, Yamashita A, Gouaux E, Science. 2008 Dec 12;322(5908):1655-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19074341 19074341]
 [[Category: Aquifex aeolicus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
+[[Category: Gouaux E]]
-[[Category: Nss]]
+[[Category: Piscitelli CL]]
-[[Category: Slc6]]
+[[Category: Singh SK]]
-[[Category: Sodium-coupled]]
+[[Category: Yamashita A]]
-[[Category: Symport]]
-[[Category: Transmembrane]]
-[[Category: Transport]]
-[[Category: Transport protein]]
-[[Category: Transporter]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 11 20:17:07 2009''